Mapping crown rust resistance at multiple time points in elite oat germplasm

Ian G. McNish, Cristiano M. Zimmer, Alexander Q. Susko, D. Jo Heuschele, Tyler Tiede, Austin J. Case, Kevin P. Smith

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Crown rust, caused by Puccinia coronata f. sp. avenae Erikss., is the most important disease impacting cultivated oat (Avena sativa L.). Genetic resistance is the most desirable management strategy. The genetic architecture of crown rust resistance is not fully understood, and previous mapping investigations have mostly ignored temporal variation. A collection of elite oat lines sourced from oat breeding programs in the American Upper Midwest and Canada was genotyped using a high-density genotyping-by-sequencing system and evaluated for crown rust disease severity at multiple time points throughout the growing season in three disease nursery environments. Genome-wide association mapping was conducted for disease severity on each observation date of each trial, area under the disease progress curve for each trial, heading date for each trial, and area under the disease progress curve in a multi-environment model. Crown rust resistance quantitative trait loci (QTL) were detected on linkage groups Mrg05, Mrg12, Mrg15, Mrg18, Mrg20, and Mrg33. None of these QTL were coincident with a days-to-heading QTL detected on Mrg02. Only the QTL detected on Mrg15 was detected in multiple mapping models. The QTL on Mrg05, Mrg12, Mrg18, Mrg20, and Mrg33 were detected on only a single observation date and were not detected on observations just days before and after. This result uncovers the importance of temporal variation in mapping experiments which is usually ignored. It is possible that high density temporal data could be used to more precisely characterize the nature of plant resistance in other systems.

Original languageEnglish (US)
Article numbere20007
JournalPlant Genome
Volume13
Issue number1
DOIs
StatePublished - Mar 1 2020

Bibliographical note

Funding Information:
The lines in the Founder population were developed by Michael S. McMullen, Frederic L. Kolb, Herbert W. Ohm, Melanie Caffe-Treml, Jim M. Legget, and Deon D. Stuthman among others. The Unified Oat Performance Nurseries were organized by Howard W. Rines. We thank Jean-Luc Jannink for launching the Public Oat Genotyping Initiative that was funded by USDA-ARS Project 8062-21000-045-00-D and enabled by laboratory assays conducted by Shiaoman Chao and bioinformatic analyses by Nick Tinker. Technical assistance was provided by Dimitri von Ruckert, Joan M. Barreto Ortiz, and Erik Boysen at the University of Minnesota? Twin Cities. We thank the researchers at the USDA-ARS Cereal Disease Laboratory in St. Paul, MN including Samantha Armintrout for critiques on this manuscript, Shahryar Kianian and Jakob Riddle for organizing the crown rust isolate survey, and Roger Caspers for managing the Matt Moore Memorial Buckthorn Nursery. Finally, we acknowledge and thank Pepsico, the Minnesota Department of Agriculture, and the Minnesota Agricultural Experiment Station (Small Grains Initiative) for funding this research.

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