Mapping adult plant stem rust resistance in barley accessions Hietpas-5 and GAW-79

Austin J. Case, Sridhar Bhavani, Godwin Macharia, Zacharias Pretorius, Vicky Coetzee, Frederik Kloppers, Priyanka Tyagi, Gina Brown-Guedira, Brian J. Steffenson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Abstract: Key messageMajor stem rust resistance QTLs proposed to beRpg2from Hietpas-5 andRpg3from GAW-79 were identified in chromosomes 2H and 5H, respectively, and will enhance the diversity of stem rust resistance in barley improvement programs. Abstract: Stem rust is a devastating disease of cereal crops worldwide. In barley (Hordeum vulgare ssp. vulgare), the disease is caused by two pathogens: Puccinia graminis f. sp. secalis (Pgs) and Puccinia graminis f. sp. tritici (Pgt). In North America, the stem rust resistance gene Rpg1 has protected barley from serious losses for more than 60 years; however, widely virulent Pgt races from Africa in the Ug99 group threaten the crop. The accessions Hietpas-5 (CIho 7124) and GAW-79 (PI 382313) both possess moderate-to-high levels of adult plant resistance to stem rust and are the sources of the resistance genes Rpg2 and Rpg3, respectively. To identify quantitative trait loci (QTL) for stem rust resistance in Hietpas-5 and GAW-79, two biparental populations were developed with Hiproly (PI 60693), a stem rust-susceptible accession. Both populations were phenotyped to the North American Pgt races of MCCFC, QCCJB, and HKHJC in St. Paul, Minnesota, and to African Pgt races (predominately TTKSK in the Ug99 group) in Njoro, Kenya. In the Hietpas-5/Hiproly population, a major effect QTL was identified in chromosome 2H, which is proposed as the location for Rpg2. In the GAW-79/Hiproly population, a major effect QTL was identified in chromosome 5H and is the proposed location for Rpg3. These QTLs will enhance the diversity of stem rust resistance in barley improvement programs.

Original languageEnglish (US)
Pages (from-to)2245-2266
Number of pages22
JournalTheoretical and Applied Genetics
Volume131
Issue number10
DOIs
StatePublished - Oct 1 2018

Bibliographical note

Funding Information:
Acknowledgements This research was funded, in part, by the Trit-iceae Coordinated Agricultural Project (2011-68002-30029) from the United States Department of Agriculture National Institute of Food and Agriculture, the Lieberman-Okinow Endowment at the University of Minnesota, American Malting Barley Association, and United States Department of Agriculture-Agricultural Research Service Cooperative Agreement 58-5062-5-012 (Understanding Stem Rust Resistance in Barley and Germplasm). AJC acknowledges financial support from the following University of Minnesota fellowships: Norman E. Bor-laug Graduate Fellowship for International Agriculture supported by the Vaale-Henry Endowment, the Minnesota Discovery, Research, and Innovation (MnDRIVE) Fellowship, and the Doctoral Dissertation Fellowship. We thank T. Szinyei and M. Martin, for excellent technical assistance, and Dr. Ahmad Sallam and Dr. María Muñoz-Amatriaín for assistance data analysis.

Funding Information:
This research was funded, in part, by the Triticeae Coordinated Agricultural Project (2011-68002-30029) from the United States Department of Agriculture National Institute of Food and Agriculture, the Lieberman-Okinow Endowment at the University of Minnesota, American Malting Barley Association, and United States Department of Agriculture-Agricultural Research Service Cooperative Agreement 58-5062-5-012 (Understanding Stem Rust Resistance in Barley and Germplasm). AJC acknowledges financial support from the following University of Minnesota fellowships: Norman E. Borlaug Graduate Fellowship for International Agriculture supported by the Vaale-Henry Endowment, the Minnesota Discovery, Research, and Innovation (MnDRIVE) Fellowship, and the Doctoral Dissertation Fellowship. We thank T. Szinyei and M. Martin, for excellent technical assistance, and Dr. Ahmad Sallam and Dr. Mar?a Mu?oz-Amatria?n for assistance data analysis. The author name Frederik Kloppers was incorrect in original publication. It has been corrected. The authors declare that they have no conflict of interest.

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