Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis

Vincent Arondel, Bertrand Lemieux, Inhwan Hwang, Sue Gibson, Howard M. Goodman, Chris R. Somerville

Research output: Contribution to journalArticle

331 Citations (Scopus)

Abstract

A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.

Original languageEnglish (US)
Pages (from-to)1353-1355
Number of pages3
JournalScience
Volume258
Issue number5086
DOIs
StatePublished - Jan 1 1992

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Omega-3 Fatty Acids
Arabidopsis
Organism Cloning
Complementary DNA
Plant Tumor-Inducing Plasmids
Fatty Acid Desaturases
Yeast Artificial Chromosomes
Plant Genes
Genetic Loci
Membrane Lipids
Gene Library
Genes
Seeds
Fatty Acids
Clone Cells
Mutation
omega-3 fatty acid desaturase

Cite this

Arondel, V., Lemieux, B., Hwang, I., Gibson, S., Goodman, H. M., & Somerville, C. R. (1992). Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science, 258(5086), 1353-1355. https://doi.org/10.1126/science.1455229

Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. / Arondel, Vincent; Lemieux, Bertrand; Hwang, Inhwan; Gibson, Sue; Goodman, Howard M.; Somerville, Chris R.

In: Science, Vol. 258, No. 5086, 01.01.1992, p. 1353-1355.

Research output: Contribution to journalArticle

Arondel, V, Lemieux, B, Hwang, I, Gibson, S, Goodman, HM & Somerville, CR 1992, 'Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis', Science, vol. 258, no. 5086, pp. 1353-1355. https://doi.org/10.1126/science.1455229
Arondel, Vincent ; Lemieux, Bertrand ; Hwang, Inhwan ; Gibson, Sue ; Goodman, Howard M. ; Somerville, Chris R. / Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. In: Science. 1992 ; Vol. 258, No. 5086. pp. 1353-1355.
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