Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis

Vincent Arondel, Bertrand Lemieux, Inhwan Hwang, Sue Gibson, Howard M. Goodman, Chris R. Somerville

Research output: Contribution to journalArticlepeer-review

390 Scopus citations

Abstract

A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.

Original languageEnglish (US)
Pages (from-to)1353-1355
Number of pages3
JournalScience
Volume258
Issue number5086
DOIs
StatePublished - 1992

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