Many Th cell subsets have fas ligand-dependent cytotoxic potential

Dmitri I. Kotov, Jessica A. Kotov, Michael F. Goldberg, Marc K. Jenkins

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


CD4 + Th cells can have cytotoxic activity against cells displaying relevant peptide-MHC class II (p:MHCII) ligands. Cytotoxicity may be a property of Th1 cells and depends on perforin and the Eomes transcription factor. We assessed these assertions for polyclonal p:MHCII-specific CD4+ T cells activated in vivo in different contexts. Mice immunized with an immunogenic peptide in adjuvant or infected with lymphocytic choriomeningitis virus or Listeria monocytogenes bacteria induced cytotoxic Th cells that killed B cells displaying relevant p:MHCII complexes. Cytotoxicity was dependent on Fas expression by target cells but was independent of Eomes or perforin expression by T cells. Although the priming regimens induced different proportions of Th1, Th17, regulatory T cells, and T follicular helper cells, the T cells expressed Fas ligand in all cases. Reciprocally, Fas was upregulated on target cells in a p:MHCII-specific manner. These results indicate that many Th subsets have cytotoxic potential that is enhanced by cognate induction of Fas on target cells.

Original languageEnglish (US)
Pages (from-to)2004-2012
Number of pages9
JournalJournal of Immunology
Issue number6
StatePublished - Mar 15 2018

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health Grants R01-AI39614, R37-AI27998, R01-AI103760, and P01-AI35296 (to M.K.J.) and T32-AI007313 and T32-AI083196 (to D.I.K.).

Publisher Copyright:
© 2018 by The American Association of Immunologists, Inc.


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