Over the past year, mammalian cell culture research has been aimed at investigating the influence of culture conditions on viability, productivity and the consistency of post-translational modifications. Studies of the effect of medium conditions and the development of kinetic models are being made in relation to current efforts to develop fed-batch strategies that will optimize recombinant protein production processes. Recent advances have included novel biosensor and bioreactor developments. New technologies have also been applied to investigate high cell density bioreactor and culture conditions.
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Part of this manuscript was prepared while Wei-Shou Hu was visiting the International Center for Cooperative Research in Biotechnology. Support from Osaka University is gratefully acknowledged. This work was supported in part by grants from the National Science Foundation, USA (BCS-8551670) to Wei-Shou Hu and from the National Science and Engineering Research Council of Canada to James Piret.