Malarial parasites complete sporogony in axenic mosquitoes

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Abstract

To obtain sporogonic stages of malaria free from microbial contaminants for in vitro studies, Anopheles stephensi were reared under sterile conditions using a mosquito cell line as larval food. The adult females, kept in sterile humidified containers and allowed to engorge on parasitemic hamsters, supported the sporogonic development of the rodent malarial parasite Plasmodium berghei. In 10 experiments, the proportion of infected mosquitoes varied from 0 to 92%, and the geometric mean number of oocysts per female mosquito from 2.5 to 58,6, with a range of 1 to 548. The average number of salivary gland sporozoites per infected mosquito was determined by direct sporozoite counts in the pooled homogenate of the thoraces of all female mosquitoes. In five experiments, it varied from 2.7×103 to 9.0×103. The sterile sporozoites, harvested on day 19 or 20 after the infective blood meal, were as infective for rodents as nonsterile ones.

Original languageEnglish (US)
Pages (from-to)1205-1207
Number of pages3
JournalExperientia
Volume41
Issue number9
DOIs
StatePublished - Sep 1985

Keywords

  • Anopheles stephensi
  • Plasmodium berghei
  • axenic mosquitoes
  • mosquito cell culture
  • transmission of malaria

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