Maize centromere mapping: A comparison of physical and genetic strategies

Ron J Okagaki, Morrison S. Jacobs, Adrian O. Stec, Ralf G. Kynast, Elizabeth Buescher, Howard W. Rines, M. Isabel Vales, Oscar Riera-Lizarazu, Martha Schneerman, Greg Doyle, Katherine L. Friedman, Rick W. Staub, David F. Weber, Terry L. Kamps, Ina F.E. Amarillo, Christine D. Chase, Hank W. Bass, Ronald L. Phillips

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Abstract

Centromere positions on 7 maize chromosomes were compared on the basis of data from 4 to 6 mapping techniques per chromosome. Centromere positions were first located relative to molecular markers by means of radiation hybrid lines and centric fission lines recovered from oat-maize chromosome addition lines. These centromere positions were then compared with new data from centric fission lines recovered from maize plants, half-tetrad mapping, and fluorescence in situ hybridizations and to data from earlier studies. Surprisingly, the choice of mapping technique was not the critical determining factor. Instead, on 4 chromosomes, results from all techniques were consistent with a single centromere position. On chromosomes 1, 3, and 6, centromere positions were not consistent even in studies using the same technique. The conflicting centromere map positions on chromosomes 1, 3, and 6 could be explained by pericentric inversions or alternative centromere positions on these chromosomes.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalJournal of Heredity
Volume99
Issue number2
DOIs
StatePublished - Mar 2008

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    Okagaki, R. J., Jacobs, M. S., Stec, A. O., Kynast, R. G., Buescher, E., Rines, H. W., Vales, M. I., Riera-Lizarazu, O., Schneerman, M., Doyle, G., Friedman, K. L., Staub, R. W., Weber, D. F., Kamps, T. L., Amarillo, I. F. E., Chase, C. D., Bass, H. W., & Phillips, R. L. (2008). Maize centromere mapping: A comparison of physical and genetic strategies. Journal of Heredity, 99(2), 85-93. https://doi.org/10.1093/jhered/esm111