Maize Carbohydrate Partitioning Defective33 Encodes an MCTP Protein and Functions in Sucrose Export from Leaves

Thu M. Tran, Tyler J. McCubbin, Saadia Bihmidine, Benjamin T. Julius, R. Frank Baker, Martin Schauflinger, Clifford Weil, Nathan Springer, Paul Chomet, Ruth Wagner, Jeff Woessner, Karen Grote, Jeanette Peevers, Thomas L. Slewinski, David M. Braun

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To sustain plant growth, development, and crop yield, sucrose must be transported from leaves to distant parts of the plant, such as seeds and roots. To identify genes that regulate sucrose accumulation and transport in maize (Zea mays), we isolated carbohydrate partitioning defective33 (cpd33), a recessive mutant that accumulated excess starch and soluble sugars in mature leaves. The cpd33 mutants also exhibited chlorosis in the leaf blades, greatly diminished plant growth, and reduced fertility. Cpd33 encodes a protein containing multiple C2 domains and transmembrane regions. Subcellular localization experiments showed the CPD33 protein localized to plasmodesmata (PD), the plasma membrane, and the endoplasmic reticulum. We also found that a loss-of-function mutant of the CPD33 homolog in Arabidopsis, QUIRKY, had a similar carbohydrate hyperaccumulation phenotype. Radioactively labeled sucrose transport assays showed that sucrose export was significantly lower in cpd33 mutant leaves relative to wild-type leaves. However, PD transport in the adaxial-abaxial direction was unaffected in cpd33 mutant leaves. Intriguingly, transmission electron microscopy revealed fewer PD at the companion cell–sieve element interface in mutant phloem tissue, providing a possible explanation for the reduced sucrose export in mutant leaves. Collectively, our results suggest that CPD33 functions to promote symplastic transport into sieve elements. Maize carbohydrate partitioning defective33 (cpd33) mutants exhibit carbohydrate hyperaccumulation in leaves, reduced sucrose export from leaves, and fewer plasmodesmata (PD) between companion cells–sieve elements in leaf minor veins. Cpd33 encodes an MCTP protein and localizes to the PD, the plasma membrane, and the endoplasmic reticulum. These data suggest Cpd33 functions to promote symplastic transport into sieve elements.

Original languageEnglish (US)
Pages (from-to)1278-1293
Number of pages16
JournalMolecular Plant
Volume12
Issue number9
DOIs
StatePublished - Sep 2 2019

Fingerprint

Plasmodesmata
Zea mays
Sucrose
Carbohydrates
sucrose
carbohydrates
plasmodesmata
corn
mutants
leaves
Proteins
proteins
Endoplasmic Reticulum
sieve elements
endoplasmic reticulum
Cell Membrane
Hypochromic Anemia
Phloem
plasma membrane
Plant Development

Keywords

  • carbohydrate accumulation
  • companion cells
  • phloem
  • plasmodesmata
  • sieve elements
  • symplastic transport

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Tran, T. M., McCubbin, T. J., Bihmidine, S., Julius, B. T., Baker, R. F., Schauflinger, M., ... Braun, D. M. (2019). Maize Carbohydrate Partitioning Defective33 Encodes an MCTP Protein and Functions in Sucrose Export from Leaves. Molecular Plant, 12(9), 1278-1293. https://doi.org/10.1016/j.molp.2019.05.001

Maize Carbohydrate Partitioning Defective33 Encodes an MCTP Protein and Functions in Sucrose Export from Leaves. / Tran, Thu M.; McCubbin, Tyler J.; Bihmidine, Saadia; Julius, Benjamin T.; Baker, R. Frank; Schauflinger, Martin; Weil, Clifford; Springer, Nathan; Chomet, Paul; Wagner, Ruth; Woessner, Jeff; Grote, Karen; Peevers, Jeanette; Slewinski, Thomas L.; Braun, David M.

In: Molecular Plant, Vol. 12, No. 9, 02.09.2019, p. 1278-1293.

Research output: Contribution to journalArticle

Tran, TM, McCubbin, TJ, Bihmidine, S, Julius, BT, Baker, RF, Schauflinger, M, Weil, C, Springer, N, Chomet, P, Wagner, R, Woessner, J, Grote, K, Peevers, J, Slewinski, TL & Braun, DM 2019, 'Maize Carbohydrate Partitioning Defective33 Encodes an MCTP Protein and Functions in Sucrose Export from Leaves', Molecular Plant, vol. 12, no. 9, pp. 1278-1293. https://doi.org/10.1016/j.molp.2019.05.001
Tran, Thu M. ; McCubbin, Tyler J. ; Bihmidine, Saadia ; Julius, Benjamin T. ; Baker, R. Frank ; Schauflinger, Martin ; Weil, Clifford ; Springer, Nathan ; Chomet, Paul ; Wagner, Ruth ; Woessner, Jeff ; Grote, Karen ; Peevers, Jeanette ; Slewinski, Thomas L. ; Braun, David M. / Maize Carbohydrate Partitioning Defective33 Encodes an MCTP Protein and Functions in Sucrose Export from Leaves. In: Molecular Plant. 2019 ; Vol. 12, No. 9. pp. 1278-1293.
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