Maintenance of pre-existing DNA methylation states through recurring excess-light stress

Diep R. Ganguly, Peter Crisp, Steven R. Eichten, Barry J. Pogson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The capacity for plant stress priming and memory and the notion of this being underpinned by DNA methylation-mediated memory is an appealing hypothesis for which there is mixed evidence. We previously established a lack of drought-induced methylome variation in Arabidopsis thaliana (Arabidopsis); however, this was tied to only minor observations of physiological memory. There are numerous independent observations demonstrating that photoprotective mechanisms, induced by excess-light stress, can lead to robust programmable changes in newly developing leaf tissues. Although key signalling molecules and transcription factors are known to promote this priming signal, an untested question is the potential involvement of chromatin marks towards the maintenance of light stress acclimation, or memory. Thus, we systematically tested our previous hypothesis of a stress-resistant methylome using a recurring excess-light stress, then analysing new, emerging, and existing tissues. The DNA methylome showed negligible stress-associated variation, with the vast majority attributable to stochastic differences. Yet, photoacclimation was evident through enhanced photosystem II performance in exposed tissues, and nonphotochemical quenching and fluorescence decline ratio showed evidence of mitotic transmission. Thus, we have observed physiological acclimation in new and emerging tissues in the absence of substantive DNA methylome changes.

Original languageEnglish (US)
Pages (from-to)1657-1672
Number of pages16
JournalPlant Cell and Environment
Volume41
Issue number7
DOIs
StatePublished - Jul 1 2018

Fingerprint

DNA methylation
DNA Methylation
Maintenance
Light
Acclimatization
Arabidopsis
acclimation
Photosystem II Protein Complex
Droughts
DNA
plant stress
photosystem II
Chromatin
chromatin
Transcription Factors
Arabidopsis thaliana
transcription factors
Fluorescence
drought
fluorescence

Keywords

  • Arabidopsis thaliana
  • NPQ
  • epigenetics
  • stress memory

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Maintenance of pre-existing DNA methylation states through recurring excess-light stress. / Ganguly, Diep R.; Crisp, Peter; Eichten, Steven R.; Pogson, Barry J.

In: Plant Cell and Environment, Vol. 41, No. 7, 01.07.2018, p. 1657-1672.

Research output: Contribution to journalArticle

Ganguly, Diep R. ; Crisp, Peter ; Eichten, Steven R. ; Pogson, Barry J. / Maintenance of pre-existing DNA methylation states through recurring excess-light stress. In: Plant Cell and Environment. 2018 ; Vol. 41, No. 7. pp. 1657-1672.
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