Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors

Atsushi Suzuki; Ángel Raya; Yasuhiko Kawakami; Masanobu Morita; Takaaki Matsui; Kinichi Nakashima; Fred H. Gage; Concepción Rodríguez-Esteban; Juan Carlos Izpisúa Belmonte

doi:10.1007/978-1-60327-227-8_4

Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors

Atsushi Suzuki
, Ángel Raya
, Yasuhiko Kawakami
, Masanobu Morita
, Takaaki Matsui
, Kinichi Nakashima
, Fred H. Gage
, Concepción Rodríguez-Esteban
, Juan Carlos Izpisúa Belmonte

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Embryonic stem (ES) cells can be propagated indefinitely in culture while retaining the ability to differentiate into any cell type in the organism. The molecular and cellular mechanisms underlying ES cell pluripotency are, however, poorly understood. Here, we characterize a population of early mesoderm-committed (EM) progenitors that is generated from mouse ES cells by bone morphogenetic protein (BMP) stimulation. We further show that EM progenitors are actively dedifferentiated to ES cells by the action of Nanog, which, in turn, is directly up-regulated in EM progenitors by the combined action of leukemia inhibitory factor (LIF) and the early mesoderm transcription factor T/Brachyury. Finally, we demonstrate that this negative feedback mechanism contributes to the maintenance of ES cell pluripotency. These findings uncover specific roles of LIF, Nanog, and BMP in the self-renewal of ES cells and provide novel insights into the cellular bases of ES cell pluripotency.

Original language	English (US)
Title of host publication	Stem Cell Biology and Regenerative Medicine
Publisher	Springer Nature
Pages	37-53
Number of pages	17
DOIs	https://doi.org/10.1007/978-1-60327-227-8_4
State	Published - 2009
Externally published	Yes

Publication series

Name	Stem Cell Biology and Regenerative Medicine
Volume	Part F4844
ISSN (Print)	2196-8985
ISSN (Electronic)	2196-8993

Bibliographical note

Publisher Copyright:
© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Leukemia inhibitory factor
Mesoderm differentiation
Pluripotency
Self-renewal
T (Brachyury)

Publisher link

10.1007/978-1-60327-227-8_4

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Suzuki, A., Raya, Á., Kawakami, Y., Morita, M., Matsui, T., Nakashima, K., Gage, F. H., Rodríguez-Esteban, C., & Belmonte, J. C. I. (2009). Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors. In Stem Cell Biology and Regenerative Medicine (pp. 37-53). (Stem Cell Biology and Regenerative Medicine; Vol. Part F4844). Springer Nature. https://doi.org/10.1007/978-1-60327-227-8_4

Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors. / Suzuki, Atsushi; Raya, Ángel; Kawakami, Yasuhiko et al.
Stem Cell Biology and Regenerative Medicine. Springer Nature, 2009. p. 37-53 (Stem Cell Biology and Regenerative Medicine; Vol. Part F4844).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Suzuki, A, Raya, Á, Kawakami, Y, Morita, M, Matsui, T, Nakashima, K, Gage, FH, Rodríguez-Esteban, C & Belmonte, JCI 2009, Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors. in Stem Cell Biology and Regenerative Medicine. Stem Cell Biology and Regenerative Medicine, vol. Part F4844, Springer Nature, pp. 37-53. https://doi.org/10.1007/978-1-60327-227-8_4

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abstract = "Embryonic stem (ES) cells can be propagated indefinitely in culture while retaining the ability to differentiate into any cell type in the organism. The molecular and cellular mechanisms underlying ES cell pluripotency are, however, poorly understood. Here, we characterize a population of early mesoderm-committed (EM) progenitors that is generated from mouse ES cells by bone morphogenetic protein (BMP) stimulation. We further show that EM progenitors are actively dedifferentiated to ES cells by the action of Nanog, which, in turn, is directly up-regulated in EM progenitors by the combined action of leukemia inhibitory factor (LIF) and the early mesoderm transcription factor T/Brachyury. Finally, we demonstrate that this negative feedback mechanism contributes to the maintenance of ES cell pluripotency. These findings uncover specific roles of LIF, Nanog, and BMP in the self-renewal of ES cells and provide novel insights into the cellular bases of ES cell pluripotency.",

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AB - Embryonic stem (ES) cells can be propagated indefinitely in culture while retaining the ability to differentiate into any cell type in the organism. The molecular and cellular mechanisms underlying ES cell pluripotency are, however, poorly understood. Here, we characterize a population of early mesoderm-committed (EM) progenitors that is generated from mouse ES cells by bone morphogenetic protein (BMP) stimulation. We further show that EM progenitors are actively dedifferentiated to ES cells by the action of Nanog, which, in turn, is directly up-regulated in EM progenitors by the combined action of leukemia inhibitory factor (LIF) and the early mesoderm transcription factor T/Brachyury. Finally, we demonstrate that this negative feedback mechanism contributes to the maintenance of ES cell pluripotency. These findings uncover specific roles of LIF, Nanog, and BMP in the self-renewal of ES cells and provide novel insights into the cellular bases of ES cell pluripotency.

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Maintenance of Embryonic Stem Cell Pluripotency by Nanog-Mediated Dedifferentiation of Committed Mesoderm Progenitors

Abstract

Publication series

Bibliographical note

UN SDGs

Keywords

Publisher link

Find It

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