Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1

Kai Wu; Jinming Liu; Renata Saha; Diqing Su; Venkatramana D. Krishna; Maxim C.J. Cheeran; Jian Ping Wang

doi:10.1021/acsami.0c00815

Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1

Kai Wu, Jinming Liu, Renata Saha, Diqing Su, Venkatramana D. Krishna, Maxim C.J. Cheeran, Jian Ping Wang

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Abstract

Magnetic nanoparticles (MNPs) with proper surface functionalization have been extensively applied as labels for magnetic immunoassays, carriers for controlled drug/gene delivery, tracers and contrasts for magnetic imaging, etc. Here, we introduce a new biosensing scheme based on magnetic particle spectroscopy (MPS) and the self-assembly of MNPs to quantitatively detect H1N1 nucleoprotein molecules. MPS monitors the harmonics of oscillating MNPs as a metric for the freedom of rotational process, thus indicating the bound states of MNPs. These harmonics can be readily collected from nanogram quantities of iron oxide nanoparticles within 10 s. The H1N1 nucleoprotein molecule hosts multiple different epitopes that forms binding sites for many IgG polyclonal antibodies. Anchoring IgG polyclonal antibodies onto MNPs triggers the cross-linking between MNPs and H1N1 nucleoprotein molecules, thereby forming MNP self-assemblies. Using MPS and the self-assembly of MNPs, we were able to detect as low as 44 nM (4.4 pmole) H1N1 nucleoprotein. In addition, the morphologies and the hydrodynamic sizes of the MNP self-assemblies are characterized to verify the MPS results. Different MNP self-assembly models such as classical cluster, open ring tetramer, and chain model as well as multimers (from dimer to pentamer) are proposed in this paper. Herein, we claim the feasibility of using MPS and the self-assembly of MNPs as a new biosensing scheme for detecting ultralow concentrations of target biomolecules, which can be employed as rapid, sensitive, and wash-free magnetic immunoassays.

Original language	English (US)
Pages (from-to)	13686-13697
Number of pages	12
Journal	ACS Applied Materials and Interfaces
Volume	12
Issue number	12
DOIs	https://doi.org/10.1021/acsami.0c00815
State	Published - Mar 25 2020

Bibliographical note

Funding Information:
This study was financially supported in part by the Institute of Engineering in Medicine of the University of Minnesota through FY18 IEM Seed Grant Funding Program and the College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA SAES General Agriculture research funds (MIN-62-097). Portions of this work were conducted in the Minnesota Nano Center, which is supported by the National Science Foundation through the National Nano Coordinated Infrastructure Network (NNCI) under Award Number ECCS-1542202. Portions of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF-funded Materials Research Facilities Network ( www.mrfn.org ) via the MRSEC program.

Publisher Copyright:
© 2020 American Chemical Society.

Keywords

influenza A virus
magnetic nanoparticles
magnetic particle spectroscopy
self-assembly
wash-free magnetic immunoassay

PubMed: MeSH publication types

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title = "Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1",

abstract = "Magnetic nanoparticles (MNPs) with proper surface functionalization have been extensively applied as labels for magnetic immunoassays, carriers for controlled drug/gene delivery, tracers and contrasts for magnetic imaging, etc. Here, we introduce a new biosensing scheme based on magnetic particle spectroscopy (MPS) and the self-assembly of MNPs to quantitatively detect H1N1 nucleoprotein molecules. MPS monitors the harmonics of oscillating MNPs as a metric for the freedom of rotational process, thus indicating the bound states of MNPs. These harmonics can be readily collected from nanogram quantities of iron oxide nanoparticles within 10 s. The H1N1 nucleoprotein molecule hosts multiple different epitopes that forms binding sites for many IgG polyclonal antibodies. Anchoring IgG polyclonal antibodies onto MNPs triggers the cross-linking between MNPs and H1N1 nucleoprotein molecules, thereby forming MNP self-assemblies. Using MPS and the self-assembly of MNPs, we were able to detect as low as 44 nM (4.4 pmole) H1N1 nucleoprotein. In addition, the morphologies and the hydrodynamic sizes of the MNP self-assemblies are characterized to verify the MPS results. Different MNP self-assembly models such as classical cluster, open ring tetramer, and chain model as well as multimers (from dimer to pentamer) are proposed in this paper. Herein, we claim the feasibility of using MPS and the self-assembly of MNPs as a new biosensing scheme for detecting ultralow concentrations of target biomolecules, which can be employed as rapid, sensitive, and wash-free magnetic immunoassays.",

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author = "Kai Wu and Jinming Liu and Renata Saha and Diqing Su and Krishna, {Venkatramana D.} and Cheeran, {Maxim C.J.} and Wang, {Jian Ping}",

note = "Funding Information: This study was financially supported in part by the Institute of Engineering in Medicine of the University of Minnesota through FY18 IEM Seed Grant Funding Program and the College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA SAES General Agriculture research funds (MIN-62-097). Portions of this work were conducted in the Minnesota Nano Center, which is supported by the National Science Foundation through the National Nano Coordinated Infrastructure Network (NNCI) under Award Number ECCS-1542202. Portions of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF-funded Materials Research Facilities Network ( www.mrfn.org ) via the MRSEC program. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

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doi = "10.1021/acsami.0c00815",

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T1 - Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1

AU - Wu, Kai

AU - Liu, Jinming

AU - Saha, Renata

AU - Su, Diqing

AU - Krishna, Venkatramana D.

AU - Cheeran, Maxim C.J.

AU - Wang, Jian Ping

N1 - Funding Information: This study was financially supported in part by the Institute of Engineering in Medicine of the University of Minnesota through FY18 IEM Seed Grant Funding Program and the College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA SAES General Agriculture research funds (MIN-62-097). Portions of this work were conducted in the Minnesota Nano Center, which is supported by the National Science Foundation through the National Nano Coordinated Infrastructure Network (NNCI) under Award Number ECCS-1542202. Portions of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF-funded Materials Research Facilities Network ( www.mrfn.org ) via the MRSEC program. Publisher Copyright: © 2020 American Chemical Society.

PY - 2020/3/25

Y1 - 2020/3/25

N2 - Magnetic nanoparticles (MNPs) with proper surface functionalization have been extensively applied as labels for magnetic immunoassays, carriers for controlled drug/gene delivery, tracers and contrasts for magnetic imaging, etc. Here, we introduce a new biosensing scheme based on magnetic particle spectroscopy (MPS) and the self-assembly of MNPs to quantitatively detect H1N1 nucleoprotein molecules. MPS monitors the harmonics of oscillating MNPs as a metric for the freedom of rotational process, thus indicating the bound states of MNPs. These harmonics can be readily collected from nanogram quantities of iron oxide nanoparticles within 10 s. The H1N1 nucleoprotein molecule hosts multiple different epitopes that forms binding sites for many IgG polyclonal antibodies. Anchoring IgG polyclonal antibodies onto MNPs triggers the cross-linking between MNPs and H1N1 nucleoprotein molecules, thereby forming MNP self-assemblies. Using MPS and the self-assembly of MNPs, we were able to detect as low as 44 nM (4.4 pmole) H1N1 nucleoprotein. In addition, the morphologies and the hydrodynamic sizes of the MNP self-assemblies are characterized to verify the MPS results. Different MNP self-assembly models such as classical cluster, open ring tetramer, and chain model as well as multimers (from dimer to pentamer) are proposed in this paper. Herein, we claim the feasibility of using MPS and the self-assembly of MNPs as a new biosensing scheme for detecting ultralow concentrations of target biomolecules, which can be employed as rapid, sensitive, and wash-free magnetic immunoassays.

AB - Magnetic nanoparticles (MNPs) with proper surface functionalization have been extensively applied as labels for magnetic immunoassays, carriers for controlled drug/gene delivery, tracers and contrasts for magnetic imaging, etc. Here, we introduce a new biosensing scheme based on magnetic particle spectroscopy (MPS) and the self-assembly of MNPs to quantitatively detect H1N1 nucleoprotein molecules. MPS monitors the harmonics of oscillating MNPs as a metric for the freedom of rotational process, thus indicating the bound states of MNPs. These harmonics can be readily collected from nanogram quantities of iron oxide nanoparticles within 10 s. The H1N1 nucleoprotein molecule hosts multiple different epitopes that forms binding sites for many IgG polyclonal antibodies. Anchoring IgG polyclonal antibodies onto MNPs triggers the cross-linking between MNPs and H1N1 nucleoprotein molecules, thereby forming MNP self-assemblies. Using MPS and the self-assembly of MNPs, we were able to detect as low as 44 nM (4.4 pmole) H1N1 nucleoprotein. In addition, the morphologies and the hydrodynamic sizes of the MNP self-assemblies are characterized to verify the MPS results. Different MNP self-assembly models such as classical cluster, open ring tetramer, and chain model as well as multimers (from dimer to pentamer) are proposed in this paper. Herein, we claim the feasibility of using MPS and the self-assembly of MNPs as a new biosensing scheme for detecting ultralow concentrations of target biomolecules, which can be employed as rapid, sensitive, and wash-free magnetic immunoassays.

KW - influenza A virus

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KW - magnetic particle spectroscopy

KW - self-assembly

KW - wash-free magnetic immunoassay

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Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1

Abstract

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