Magnesium antagonizes the actions of lysophosphatidyl choline (LPC) in myocardial cells: A possible mechanism for its antiarrhythmic effects

R. C. Prielipp, J. F. Butterworth IV, P. R. Roberts, K. W. Black, G. P. Zaloga

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Patients with cardiac arrhythmias, ischemia, and infarction may benefit from administration of supplemental magnesium. However, the exact mechanisms for magnesium's beneficial effects remain unknown. Lysophosphatidyl choline (LPC), an amphipathic phospholipid released from cardiac cell membranes during ischemia, increases free intracellular calcium concentrations ([Ca](i)) and has been implicated as a cause of cardiac arrhythmias and coronary artery spasm during myocardial ischemia. We postulated that magnesium acts by inhibiting cellular calcium overload induced by mediators such as LPC. Myocardial cells from male Sprague-Dawley rats were isolated from ventricular tissue samples and [Ca](i) determined using the fluorescent dye, fura-2/acetoxymethyl ester, measured in a spectrofluorometer. The increase in [Ca](i) after exposure to 100 and 200 μM LPC was recorded in cells suspended in modified Dulbecco's phosphate buffered saline solution with 0.2, 2.0, and 20 mM magnesium chloride. Differences were determined by analysis of variance with P < 0.05 considered significant. LPC significantly increased [Ca](i) in the 100 μM (506 ± 76 nM) and 200 μM (675 ± 81 nM) concentrations, compared to baseline (301 ± 25 nM). MgCl2 at both the 2.0 and 20 mM concentrations significantly blurt ted the increase in [Ca](i) in myocardial cells exposed to LPC, whereas 0.2 mM MgCl2 was ineffective. LPC is a potent lipid mediator which increases myocyte [Ca](i) in a concentration-dependent manner. Magnesium concentrations ≥2.0 mM effectively antagonize the increase in [Ca](i) induced by LPC. Thus, magnesium may limit intracellular calcium overload stimulated by ischemic-induced LPC release.

Original languageEnglish (US)
Pages (from-to)1083-1087
Number of pages5
JournalAnesthesia and analgesia
Volume80
Issue number6
DOIs
StatePublished - Jun 12 1995

Fingerprint Dive into the research topics of 'Magnesium antagonizes the actions of lysophosphatidyl choline (LPC) in myocardial cells: A possible mechanism for its antiarrhythmic effects'. Together they form a unique fingerprint.

Cite this