Macromolecular replication during lignin biosynthesis

Yi ru Chen, Simo Sarkanen

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Lignins play a crucial role in the cell-wall architecture of all vascular plants. They are composed of p-hydroxyphenylpropanoid units interconnected through covalent bonds formed during lignol radical coupling between six different pairs of atomic centers. For 50 years, the primary structures of lignins have been thought to be random, but for a number of reasons such an assumption is not tenable. For example, it has been reported that, by simple physicochemical means, the rather recalcitrant lignins in spruce wood can be decisively separated into two fractions containing quite dissimilar biopolymer chains. Thus, a paradigm shift should be imminent, and a detailed working hypothesis for the mechanism of lignin biosynthesis would be invaluable in delineating how the process of macromolecular lignin assembly can be properly investigated. In conjunction with an earlier experimental result, an explicit model for a template dehydropolymerization process has been developed that describes how lignin primary structure is replicated. The strengths of the powerful noncovalent interactions have been calculated that control the transient placement of lignol radicals about to undergo coupling on a double-stranded lignin template. These elementary steps engender, in the growing daughter chain, a primary structure identical to that of the distal template strand. The interactions are governed by dynamical electron correlation in the π-orbitals of each immobilized lignol radical and the complementary aromatic ring in the antiparallel proximal strand. The resulting noncovalent forces are computed to be stronger than those stabilizing GC/CG base pairs in DNA double-helices, but the mechanism of replication is fundamentally different from that of any other biopolymer.

Original languageEnglish (US)
Pages (from-to)453-462
Number of pages10
JournalPhytochemistry
Volume71
Issue number4
DOIs
StatePublished - Mar 2010

Bibliographical note

Funding Information:
The authors express their gratitude for the computational resources provided by the University of Minnesota Supercomputing Institute. Hatch funds and a subcontract from the BioEnergy Science Center, which is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science, are gratefully acknowledged.

Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.

Keywords

  • Density functional theory
  • Dynamical electron correlation
  • Lignin primary structure
  • Replication
  • Template polymerization

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