Lysophosphatidic acid stimulates calcium transients in enteric glia

B. J. Segura, W. Zhang, R. A. Cowles, L. Xiao, T. R. Lin, C. Logsdon, M. W. Mulholland

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The enteric nervous system plays an integral role in the gastrointestinal tract. Within this intricate network, enteric glia are crucial in the maintenance of normal bowel function, yet their signaling mechanisms are poorly understood. Enteric glia, and not enteric neurons, selectively responded to lysophosphatidic acid (LPA), a product of phosphatidylcholine metabolism, with dose-dependent calcium (Ca2+) signaling over a range from 100 pM to 10 μM. The elicited calcium transients involved both the mobilization of intracellular Ca2+ stores and the influx of extracellular Ca 2+ as LPA signals were obliterated following the depletion of intracellular Ca2+ and attenuated by the removal of Ca2+ from the perfusion buffer. Pretreatment with pertussis toxin (100 ng/ml) reduced the magnitude of LPA Ca2+ transients (95±20 nM vs 168±17 nM for controls). Repetitive exposure yielded diminished responsiveness, with a 25% reduction in [Ca2+]i between first and second exposures. Inhibition of the inositol 1,4,5-trisphosphate (IP3) receptor with 200 μM 2-aminoethoxydiphenylborate (2APB) abolished LPA signals. RT-PCR analysis demonstrated the presence of two LPA-coupled endothelial differentiation gene (EDG) receptor mRNAs (EDG-2 and EDG-7) in myenteric plexus primary cultures. EDG-2 expression in glial cells of the ENS was confirmed immunocytochemically.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalNeuroscience
Volume123
Issue number3
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Keywords

  • EDG receptors
  • Enteric nervous system
  • Sphingolipid

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    Segura, B. J., Zhang, W., Cowles, R. A., Xiao, L., Lin, T. R., Logsdon, C., & Mulholland, M. W. (2004). Lysophosphatidic acid stimulates calcium transients in enteric glia. Neuroscience, 123(3), 687-693. https://doi.org/10.1016/j.neuroscience.2003.10.003