Primary hypoadrenocorticism, or Addison’s disease, is an autoimmune condition common in certain dog breeds that leads to the destruction of the adrenal cortex and a clinical syndrome involving anorexia, gastrointestinal upset, and electrolyte imbalances. Previous studies have demonstrated that this destruction is strongly associated with lymphocytic-plasmacytic inflammation and that the lymphocytes are primarily T cells. In this study, we used both immunohistochemistry and in situ hybridization to characterize the T-cell subtypes involved. We collected postmortem specimens of 5 dogs with primary hypoadrenocorticism and 2 control dogs and, using the aforementioned techniques, showed that the lymphocytes are primarily CD4+ rather than CD8+. These findings have important implications for improving our understanding of the pathogenesis and in searching for the underlying causative genetic polymorphisms.
Bibliographical noteFunding Information:
The author(s) received the following financial support for the research, authorship, and/or publication of this article: This study was funded in part by a National Institutes of Health T32 training award provided to SGF (5T32OD11130-07). Additional funding was provided by the American Kennel Club Canine Health Foundation and the Poodle Club of America Foundation.
© 2016, © The Author(s) 2016.
Copyright 2018 Elsevier B.V., All rights reserved.
- Addison’s disease
- adrenal gland
- endocrine diseases
- lymphocyte subsets