LPS induces GROα chemokine production via NF-κB in oral fibroblasts

D. Jönsson, S. Amisten, G. Bratthall, A. Holm, Bengt Olof Nilsson

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Objective and design: Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROα, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROα by oral fibroblasts have not previously been presented. Materials and methods: GROα mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. Results: We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROα in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROα and GROα protein. The steroid hormone estrogen had no effect on LPS-induced GROα mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROα production, and the NF-κB blocker MG 132 fully prevented LPS-induced GROα. Conclusions: Oral fibroblasts respond to LPS stimulation by increasing GROα production via the transcription factor NF-κB, suggesting that this mechanism may be involved in development of periodontal inflammation.

Original languageEnglish (US)
Pages (from-to)791-796
Number of pages6
JournalInflammation Research
Volume58
Issue number11
DOIs
StatePublished - Nov 1 2009

Keywords

  • Chemokines
  • Dexamethasone
  • Estrogen
  • GROα
  • PDL cells

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