Objective and design: Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROα, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROα by oral fibroblasts have not previously been presented. Materials and methods: GROα mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. Results: We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROα in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROα and GROα protein. The steroid hormone estrogen had no effect on LPS-induced GROα mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROα production, and the NF-κB blocker MG 132 fully prevented LPS-induced GROα. Conclusions: Oral fibroblasts respond to LPS stimulation by increasing GROα production via the transcription factor NF-κB, suggesting that this mechanism may be involved in development of periodontal inflammation.
- PDL cells