Nizkomolekuliarnye tsitoliziny i ingibitory tripsina iz morskoi aktinii Radianthus macrodactylus. Vydelenie i chastichnaia kharakteristika.

Translated title of the contribution: Low-molecular cytolysins and trypsin inhibitors from sea anemone Radianthus macrodactylus. Isolation and partial characterization

T. A. Zykova, M. M. Monastyrnaia, O. V. Apalikova, T. V. Shvets, E. P. Kozlovskaia

Research output: Contribution to journalArticlepeer-review

Abstract

Two low-molecular cytolytic toxins (RmI and RmII) and four trypsin inhibitors were isolated from the aqueous extract of sea anemone Radianthus macrodactylus. The method of isolation involved precipitation with acetone, gel filtration on acrylex P-4, ion-exchange chromatography on CM-32 cellulose, affinity chromatography on trypsin-binding sepharose 4B, ion exchange chromatography on an Ultrapore TSK CM-3SW column, and reversed phase HPLC on a Silasorb C18 column. RmI, RmII, and JnI inhibitor displayed molecular masses 5100, 6100, and 7100 Da, respectively, when subjected to SDS-PAGE. The isoelectric points were 9.2 and 9.3 for RmI and RmII, respectively. The amino acid composition and N-terminal amino acid residue (glycine) were determined for RmI, RmII, and JnI. Both proteins were nontoxic to mice and crabs. Hemolytic activity was determined to be 25 and 20 HU/mg for RmI and RmII, respectively, and their action on erythrocyte membrane was not inhibited by exogenous sphingomyelin. RmI and RmII exhibited antihistamine activity.

Translated title of the contributionLow-molecular cytolysins and trypsin inhibitors from sea anemone Radianthus macrodactylus. Isolation and partial characterization
Original languageRussian
Pages (from-to)509-516
Number of pages8
JournalBioorganicheskaia khimiia
Volume24
Issue number7
StatePublished - Jul 1 1998

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