Low-Fat, High-Fiber Diet Reduces Markers of Inflammation and Dysbiosis and Improves Quality of Life in Patients With Ulcerative Colitis

Julia Fritsch, Luis Garces, Maria A. Quintero, Judith Pignac-Kobinger, Ana M. Santander, Irina Fernández, Yuguang J. Ban, Deukwoo Kwon, Matthew C. Phillips, Karina Knight, Qingqing Mao, Rebeca Santaolalla, Xi S. Chen, Mukil Maruthamuthu, Norma Solis, Oriana M. Damas, David H. Kerman, Amar R. Deshpande, John E. Lewis, Chi ChenMaria T. Abreu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background & Aims: A high-fat diet has been associated with an increased risk of ulcerative colitis (UC). We studied the effects of a low-fat, high-fiber diet (LFD) vs an improved standard American diet (iSAD, included higher quantities of fruits, vegetables, and fiber than a typical SAD). We collected data on quality of life, markers of inflammation, and fecal markers of intestinal dysbiosis in patients with UC. Methods: We analyzed data from a parallel-group, cross-over study of 17 patients with UC in remission or with mild disease (with a flare within the past 18 mo), from February 25, 2015, through September 11, 2018. Participants were assigned randomly to 2 groups and received a LFD (10% of calories from fat) or an iSAD (35%–40% of calories from fat) for the first 4-week period, followed by a 2-week washout period, and then switched to the other diet for 4 weeks. All diets were catered and delivered to patients’ homes, and each participant served as her or his own control. Serum and stool samples were collected at baseline and week 4 of each diet and analyzed for markers of inflammation. We performed 16s ribosomal RNA sequencing and untargeted and targeted metabolomic analyses on stool samples. The primary outcome was quality of life, which was measured by the short inflammatory bowel disease (IBD) questionnaire at baseline and week 4 of the diets. Secondary outcomes included changes in the Short-Form 36 health survey, partial Mayo score, markers of inflammation, microbiome and metabolome analysis, and adherence to the diet. Results: Participants’ baseline diets were unhealthier than either study diet. All patients remained in remission throughout the study period. Compared with baseline, the iSAD and LFD each increased quality of life, based on the short IBD questionnaire and Short-Form 36 health survey scores (baseline short IBD questionnaire score, 4.98; iSAD, 5.55; LFD, 5.77; baseline vs iSAD, P = .02; baseline vs LFD, P = .001). Serum amyloid A decreased significantly from 7.99 mg/L at baseline to 4.50 mg/L after LFD (P = .02), but did not decrease significantly compared with iSAD (7.20 mg/L; iSAD vs LFD, P = .07). The serum level of C-reactive protein decreased numerically from 3.23 mg/L at baseline to 2.51 mg/L after LFD (P = .07). The relative abundance of Actinobacteria in fecal samples decreased from 13.69% at baseline to 7.82% after LFD (P = .017), whereas the relative abundance of Bacteroidetes increased from 14.6% at baseline to 24.02% on LFD (P = .015). The relative abundance of Faecalibacterium prausnitzii was higher after 4 weeks on the LFD (7.20%) compared with iSAD (5.37%; P = .04). Fecal levels of acetate (an anti-inflammatory metabolite) increased from a relative abundance of 40.37 at baseline to 42.52 on the iSAD and 53.98 on the LFD (baseline vs LFD, P = .05; iSAD vs LFD, P = .09). The fecal level of tryptophan decreased from a relative abundance of 1.33 at baseline to 1.08 on the iSAD (P = .43), but increased to a relative abundance of 2.27 on the LFD (baseline vs LFD, P = .04; iSAD vs LFD, P = .08); fecal levels of lauric acid decreased after LFD (baseline, 203.4; iSAD, 381.4; LFD, 29.91; baseline vs LFD, P = .04; iSAD vs LFD, P = .02). Conclusions: In a cross-over study of patients with UC in remission, we found that a catered LFD or iSAD were each well tolerated and increased quality of life. However, the LFD decreased markers of inflammation and reduced intestinal dysbiosis in fecal samples. Dietary interventions therefore might benefit patients with UC in remission. ClinicalTrials.gov no: NCT04147598.

Original languageEnglish (US)
JournalClinical Gastroenterology and Hepatology
DOIs
StateAccepted/In press - 2020

Bibliographical note

Funding Information:
Funding This work was supported by the Crohn’s and Colitis Foundation Broad Medical Research Program (Litwin IBD Pioneers Initiative; IBD-0389R), the Micky and Madeleine Arison Family Foundation Crohn’s and Colitis Discovery Laboratory, and the Martin Kalser Chair (M.T.A.).

Funding Information:
The authors thank Melissa Kaplan and Joanna Lopez for their guidance in generating diet plans, and Elisa Karhu for her help in organizing the clinical tables. CRediT Authorship Contribtuions, Julia Fritsch (Conceptualization: Lead; Data curation: Lead; Formal analysis: Lead; Investigation: Lead; Software: Equal; Validation: Equal; Visualization: Lead; Writing ? original draft: Lead; Writing ? review & editing: Lead);, Luis Garces (Data curation: Supporting; Dietitian, recruited patients, acquired patient data and samples: Lead);, Maria A. Quintero (Data curation: Supporting; Project administration: Equal; Clinical coordinator, recruited patients, acquired patient data and samples: Lead);, Judith Pignac-Kobinger (Data curation: Supporting; Project administration: Lead; Writing ? review & editing: Supporting);, Ana M. Santander (Investigation: Equal);, Irina Fern?ndez (Investigation: Equal);, Yuguang J. Ban (Software: Equal; Writing ? review & editing: Supporting);, Deukwoo Kwon (Formal analysis: Supporting; Statistical analysis: Lead);, Matthew C. Phillips (Conceptualization: Supporting);, Karina Knight (Dietitian: Supporting);, Qingqing Mao (Metabolite analysis: Equal);, Rebeca Santaolalla (Conceptualization: Supporting; Methodology: Supporting);, Xi S. Chen (Statistical Analysis: Equal);, Mukil Maruthamuthu (Metabolite analysis: Supporting);, Norma Solis (Acquired patient data and samples: Supporting);, Oriana M. Damas (Acquired patient data and samples: Supporting);, David H. Kerman (Resources: Supporting; Acquired patient data and samples: Supporting);, Amar R. Deshpande (Resources: Supporting; Acquired patient data and samples: Supporting);, John E. Lewis (Conceptualization: Supporting; Methodology: Supporting; Validation: Supporting);, Chi Chen (Performed metabolomics and analyzed metabolite data: Lead);, Maria T. Abreu (Conceptualization: Lead; Funding acquisition: Lead; Methodology: Lead; Resources: Lead; Supervision: Lead; Validation: Lead; Writing ? review & editing: Lead). Conflicts of interest This author discloses the following: Maria T. Abreu has served as a scientific advisory board member for Boehringer Ingelheim Pharmaceuticals, Gilead, AbbVie, Seres Therapeutics, Shire, and Landos Biopharma, serves as a trainer or lecturer for Imedex, Focus Medical Communications, and Cornerstones Health, Inc, has served as a consultant for Ferring Pharmaceuticals, Allergan, Amgen, Celltrion Healthcare CO, Millennium Pharmaceuticals, Theravance Biopharma, Inc, and UCB Biopharma SRL, and has funded projects by Pfizer, Prometheus Laboratories, and Takeda Pharmaceuticals. The remaining authors disclose no conflicts. Funding This work was supported by the Crohn's and Colitis Foundation Broad Medical Research Program (Litwin IBD Pioneers Initiative; IBD-0389R), the Micky and Madeleine Arison Family Foundation Crohn's and Colitis Discovery Laboratory, and the Martin Kalser Chair (M.T.A.).

Publisher Copyright:
© 2020 The Authors

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • Lifestyle Adjustment
  • Metabolites
  • Microbiota
  • Western Diet

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Low-Fat, High-Fiber Diet Reduces Markers of Inflammation and Dysbiosis and Improves Quality of Life in Patients With Ulcerative Colitis'. Together they form a unique fingerprint.

Cite this