In the cultivation of hybridoma cells HB8178 in suspension, exponential growth was followed by cell death after reaching a maximum cell concentration. We examined the cause of such transition from growth to death. Exogenously added lactate and ammonium did not cause cell death at the concentrations observed at the end of exponential growth. The rate of cell death decreases by diluting the conditioned medium with phosphate buffer saline, suggesting the presence of inhibitory factor(s) in the conditioned medium. This inhibitory factor(s) is dialyzable. Furthermore, the conditioned medium obtained from HB8178 culture also causes transition to death phase for another hybridoma cell line AFP.
Bibliographical noteFunding Information:
This work was supported m part by grants from Economics Laboratory, St Paul, MN and from the National Science Foundation (ECE-8512427 and ECE-8552670) GYJ was supported by a fellowship from the government of the People’s Republic of China The authors thank Drs George Wang and Pete Sadowskl for useful discussions
- cell culture
- growth inhibition