Loss of viability in hybridoma cell culture-A kinetic study

Timothy C. Dodge; Guang Yong Ji; Wei Shou Hu

doi:10.1016/0141-0229(87)90114-1

Loss of viability in hybridoma cell culture-A kinetic study

Timothy C. Dodge, Guang Yong Ji, Wei Shou Hu

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

In the cultivation of hybridoma cells HB8178 in suspension, exponential growth was followed by cell death after reaching a maximum cell concentration. We examined the cause of such transition from growth to death. Exogenously added lactate and ammonium did not cause cell death at the concentrations observed at the end of exponential growth. The rate of cell death decreases by diluting the conditioned medium with phosphate buffer saline, suggesting the presence of inhibitory factor(s) in the conditioned medium. This inhibitory factor(s) is dialyzable. Furthermore, the conditioned medium obtained from HB8178 culture also causes transition to death phase for another hybridoma cell line AFP.

Original language	English (US)
Pages (from-to)	607-611
Number of pages	5
Journal	Enzyme and Microbial Technology
Volume	9
Issue number	10
DOIs	https://doi.org/10.1016/0141-0229(87)90114-1
State	Published - Oct 1987

Bibliographical note

Funding Information:
This work was supported m part by grants from Economics Laboratory, St Paul, MN and from the National Science Foundation (ECE-8512427 and ECE-8552670) GYJ was supported by a fellowship from the government of the People’s Republic of China The authors thank Drs George Wang and Pete Sadowskl for useful discussions

Keywords

cell culture
growth inhibition
hybridoma

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10.1016/0141-0229(87)90114-1

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title = "Loss of viability in hybridoma cell culture-A kinetic study",

abstract = "In the cultivation of hybridoma cells HB8178 in suspension, exponential growth was followed by cell death after reaching a maximum cell concentration. We examined the cause of such transition from growth to death. Exogenously added lactate and ammonium did not cause cell death at the concentrations observed at the end of exponential growth. The rate of cell death decreases by diluting the conditioned medium with phosphate buffer saline, suggesting the presence of inhibitory factor(s) in the conditioned medium. This inhibitory factor(s) is dialyzable. Furthermore, the conditioned medium obtained from HB8178 culture also causes transition to death phase for another hybridoma cell line AFP.",

keywords = "cell culture, growth inhibition, hybridoma",

author = "Dodge, {Timothy C.} and Ji, {Guang Yong} and Hu, {Wei Shou}",

note = "Funding Information: This work was supported m part by grants from Economics Laboratory, St Paul, MN and from the National Science Foundation (ECE-8512427 and ECE-8552670) GYJ was supported by a fellowship from the government of the People{\textquoteright}s Republic of China The authors thank Drs George Wang and Pete Sadowskl for useful discussions",

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doi = "10.1016/0141-0229(87)90114-1",

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T1 - Loss of viability in hybridoma cell culture-A kinetic study

AU - Dodge, Timothy C.

AU - Ji, Guang Yong

AU - Hu, Wei Shou

N1 - Funding Information: This work was supported m part by grants from Economics Laboratory, St Paul, MN and from the National Science Foundation (ECE-8512427 and ECE-8552670) GYJ was supported by a fellowship from the government of the People’s Republic of China The authors thank Drs George Wang and Pete Sadowskl for useful discussions

PY - 1987/10

Y1 - 1987/10

N2 - In the cultivation of hybridoma cells HB8178 in suspension, exponential growth was followed by cell death after reaching a maximum cell concentration. We examined the cause of such transition from growth to death. Exogenously added lactate and ammonium did not cause cell death at the concentrations observed at the end of exponential growth. The rate of cell death decreases by diluting the conditioned medium with phosphate buffer saline, suggesting the presence of inhibitory factor(s) in the conditioned medium. This inhibitory factor(s) is dialyzable. Furthermore, the conditioned medium obtained from HB8178 culture also causes transition to death phase for another hybridoma cell line AFP.

AB - In the cultivation of hybridoma cells HB8178 in suspension, exponential growth was followed by cell death after reaching a maximum cell concentration. We examined the cause of such transition from growth to death. Exogenously added lactate and ammonium did not cause cell death at the concentrations observed at the end of exponential growth. The rate of cell death decreases by diluting the conditioned medium with phosphate buffer saline, suggesting the presence of inhibitory factor(s) in the conditioned medium. This inhibitory factor(s) is dialyzable. Furthermore, the conditioned medium obtained from HB8178 culture also causes transition to death phase for another hybridoma cell line AFP.

KW - cell culture

KW - growth inhibition

KW - hybridoma

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SP - 607

EP - 611

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 10

ER -

Loss of viability in hybridoma cell culture-A kinetic study

Abstract

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