Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration

Szabolcs Felszeghy, Johanna Viiri, Jussi J. Paterno, Juha M.T. Hyttinen, Ali Koskela, Mei Chen, Henri Leinonen, Heikki Tanila, Niko Kivinen, Arto Koistinen, Elisa Toropainen, Marialaura Amadio, Adrian Smedowski, Mika Reinisalo, Mateusz Winiarczyk, Jerzy Mackiewicz, Maija Mutikainen, Anna Kaisa Ruotsalainen, Mikko Kettunen, Kimmo JokivarsiDebasish Sinha, Kati Kinnunen, Goran Petrovski, Janusz Blasiak, Geir Bjørkøy, Ari Koskelainen, Heli Skottman, Arto Urtti, Antero Salminen, Ram Kannan, Deborah A. Ferrington, Heping Xu, Anna Liisa Levonen, Pasi Tavi, Anu Kauppinen, Kai Kaarniranta

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Age-related macular degeneration (AMD) is a multi-factorial disease that is the leading cause of irreversible and severe vision loss in the developed countries. It has been suggested that the pathogenesis of dry AMD involves impaired protein degradation in retinal pigment epithelial cells (RPE). RPE cells are constantly exposed to oxidative stress that may lead to the accumulation of damaged cellular proteins, DNA and lipids and evoke tissue deterioration during the aging process. The ubiquitin-proteasome pathway and the lysosomal/autophagosomal pathway are the two major proteolytic systems in eukaryotic cells. NRF-2 (nuclear factor-erythroid 2-related factor-2) and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1 alpha) are master transcription factors in the regulation of cellular detoxification. We investigated the role of NRF-2 and PGC-1α in the regulation of RPE cell structure and function by using global double knockout (dKO) mice. The NRF-2/PGC-1α dKO mice exhibited significant age-dependent RPE degeneration, accumulation of the oxidative stress marker, 4-HNE (4-hydroxynonenal), the endoplasmic reticulum stress markers GRP78 (glucose-regulated protein 78) and ATF4 (activating transcription factor 4), and damaged mitochondria. Moreover, levels of protein ubiquitination and autophagy markers p62/SQSTM1 (sequestosome 1), Beclin-1 and LC3B (microtubule associated protein 1 light chain 3 beta) were significantly increased together with the Iba-1 (ionized calcium binding adaptor molecule 1) mononuclear phagocyte marker and an enlargement of RPE size. These histopathological changes of RPE were accompanied by photoreceptor dysmorphology and vision loss as revealed by electroretinography. Consequently, these novel findings suggest that the NRF-2/PGC-1α dKO mouse is a valuable model for investigating the role of proteasomal and autophagy clearance in the RPE and in the development of dry AMD.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalRedox Biology
Volume20
DOIs
StatePublished - Jan 2019

Fingerprint

Retinal Pigments
Retinal Pigment Epithelium
Macular Degeneration
Genes
Epithelial Cells
Knockout Mice
Oxidative stress
Autophagy
Oxidative Stress
Activating Transcription Factor 4
Electroretinography
Detoxification
Proteins
Mitochondria
Endoplasmic Reticulum Stress
Microtubule-Associated Proteins
Ubiquitination
Eukaryotic Cells
Proteasome Endopeptidase Complex
Phagocytes

Keywords

  • Aging
  • Autophagy
  • Degeneration
  • Oxidative stress
  • Proteasome
  • Protein aggregation

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Felszeghy, S., Viiri, J., Paterno, J. J., Hyttinen, J. M. T., Koskela, A., Chen, M., ... Kaarniranta, K. (2019). Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration. Redox Biology, 20, 1-12. https://doi.org/10.1016/j.redox.2018.09.011

Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration. / Felszeghy, Szabolcs; Viiri, Johanna; Paterno, Jussi J.; Hyttinen, Juha M.T.; Koskela, Ali; Chen, Mei; Leinonen, Henri; Tanila, Heikki; Kivinen, Niko; Koistinen, Arto; Toropainen, Elisa; Amadio, Marialaura; Smedowski, Adrian; Reinisalo, Mika; Winiarczyk, Mateusz; Mackiewicz, Jerzy; Mutikainen, Maija; Ruotsalainen, Anna Kaisa; Kettunen, Mikko; Jokivarsi, Kimmo; Sinha, Debasish; Kinnunen, Kati; Petrovski, Goran; Blasiak, Janusz; Bjørkøy, Geir; Koskelainen, Ari; Skottman, Heli; Urtti, Arto; Salminen, Antero; Kannan, Ram; Ferrington, Deborah A.; Xu, Heping; Levonen, Anna Liisa; Tavi, Pasi; Kauppinen, Anu; Kaarniranta, Kai.

In: Redox Biology, Vol. 20, 01.2019, p. 1-12.

Research output: Contribution to journalArticle

Felszeghy, S, Viiri, J, Paterno, JJ, Hyttinen, JMT, Koskela, A, Chen, M, Leinonen, H, Tanila, H, Kivinen, N, Koistinen, A, Toropainen, E, Amadio, M, Smedowski, A, Reinisalo, M, Winiarczyk, M, Mackiewicz, J, Mutikainen, M, Ruotsalainen, AK, Kettunen, M, Jokivarsi, K, Sinha, D, Kinnunen, K, Petrovski, G, Blasiak, J, Bjørkøy, G, Koskelainen, A, Skottman, H, Urtti, A, Salminen, A, Kannan, R, Ferrington, DA, Xu, H, Levonen, AL, Tavi, P, Kauppinen, A & Kaarniranta, K 2019, 'Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration', Redox Biology, vol. 20, pp. 1-12. https://doi.org/10.1016/j.redox.2018.09.011
Felszeghy, Szabolcs ; Viiri, Johanna ; Paterno, Jussi J. ; Hyttinen, Juha M.T. ; Koskela, Ali ; Chen, Mei ; Leinonen, Henri ; Tanila, Heikki ; Kivinen, Niko ; Koistinen, Arto ; Toropainen, Elisa ; Amadio, Marialaura ; Smedowski, Adrian ; Reinisalo, Mika ; Winiarczyk, Mateusz ; Mackiewicz, Jerzy ; Mutikainen, Maija ; Ruotsalainen, Anna Kaisa ; Kettunen, Mikko ; Jokivarsi, Kimmo ; Sinha, Debasish ; Kinnunen, Kati ; Petrovski, Goran ; Blasiak, Janusz ; Bjørkøy, Geir ; Koskelainen, Ari ; Skottman, Heli ; Urtti, Arto ; Salminen, Antero ; Kannan, Ram ; Ferrington, Deborah A. ; Xu, Heping ; Levonen, Anna Liisa ; Tavi, Pasi ; Kauppinen, Anu ; Kaarniranta, Kai. / Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration. In: Redox Biology. 2019 ; Vol. 20. pp. 1-12.
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abstract = "Age-related macular degeneration (AMD) is a multi-factorial disease that is the leading cause of irreversible and severe vision loss in the developed countries. It has been suggested that the pathogenesis of dry AMD involves impaired protein degradation in retinal pigment epithelial cells (RPE). RPE cells are constantly exposed to oxidative stress that may lead to the accumulation of damaged cellular proteins, DNA and lipids and evoke tissue deterioration during the aging process. The ubiquitin-proteasome pathway and the lysosomal/autophagosomal pathway are the two major proteolytic systems in eukaryotic cells. NRF-2 (nuclear factor-erythroid 2-related factor-2) and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1 alpha) are master transcription factors in the regulation of cellular detoxification. We investigated the role of NRF-2 and PGC-1α in the regulation of RPE cell structure and function by using global double knockout (dKO) mice. The NRF-2/PGC-1α dKO mice exhibited significant age-dependent RPE degeneration, accumulation of the oxidative stress marker, 4-HNE (4-hydroxynonenal), the endoplasmic reticulum stress markers GRP78 (glucose-regulated protein 78) and ATF4 (activating transcription factor 4), and damaged mitochondria. Moreover, levels of protein ubiquitination and autophagy markers p62/SQSTM1 (sequestosome 1), Beclin-1 and LC3B (microtubule associated protein 1 light chain 3 beta) were significantly increased together with the Iba-1 (ionized calcium binding adaptor molecule 1) mononuclear phagocyte marker and an enlargement of RPE size. These histopathological changes of RPE were accompanied by photoreceptor dysmorphology and vision loss as revealed by electroretinography. Consequently, these novel findings suggest that the NRF-2/PGC-1α dKO mouse is a valuable model for investigating the role of proteasomal and autophagy clearance in the RPE and in the development of dry AMD.",
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T1 - Loss of NRF-2 and PGC-1α genes leads to retinal pigment epithelium damage resembling dry age-related macular degeneration

AU - Felszeghy, Szabolcs

AU - Viiri, Johanna

AU - Paterno, Jussi J.

AU - Hyttinen, Juha M.T.

AU - Koskela, Ali

AU - Chen, Mei

AU - Leinonen, Henri

AU - Tanila, Heikki

AU - Kivinen, Niko

AU - Koistinen, Arto

AU - Toropainen, Elisa

AU - Amadio, Marialaura

AU - Smedowski, Adrian

AU - Reinisalo, Mika

AU - Winiarczyk, Mateusz

AU - Mackiewicz, Jerzy

AU - Mutikainen, Maija

AU - Ruotsalainen, Anna Kaisa

AU - Kettunen, Mikko

AU - Jokivarsi, Kimmo

AU - Sinha, Debasish

AU - Kinnunen, Kati

AU - Petrovski, Goran

AU - Blasiak, Janusz

AU - Bjørkøy, Geir

AU - Koskelainen, Ari

AU - Skottman, Heli

AU - Urtti, Arto

AU - Salminen, Antero

AU - Kannan, Ram

AU - Ferrington, Deborah A.

AU - Xu, Heping

AU - Levonen, Anna Liisa

AU - Tavi, Pasi

AU - Kauppinen, Anu

AU - Kaarniranta, Kai

PY - 2019/1

Y1 - 2019/1

N2 - Age-related macular degeneration (AMD) is a multi-factorial disease that is the leading cause of irreversible and severe vision loss in the developed countries. It has been suggested that the pathogenesis of dry AMD involves impaired protein degradation in retinal pigment epithelial cells (RPE). RPE cells are constantly exposed to oxidative stress that may lead to the accumulation of damaged cellular proteins, DNA and lipids and evoke tissue deterioration during the aging process. The ubiquitin-proteasome pathway and the lysosomal/autophagosomal pathway are the two major proteolytic systems in eukaryotic cells. NRF-2 (nuclear factor-erythroid 2-related factor-2) and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1 alpha) are master transcription factors in the regulation of cellular detoxification. We investigated the role of NRF-2 and PGC-1α in the regulation of RPE cell structure and function by using global double knockout (dKO) mice. The NRF-2/PGC-1α dKO mice exhibited significant age-dependent RPE degeneration, accumulation of the oxidative stress marker, 4-HNE (4-hydroxynonenal), the endoplasmic reticulum stress markers GRP78 (glucose-regulated protein 78) and ATF4 (activating transcription factor 4), and damaged mitochondria. Moreover, levels of protein ubiquitination and autophagy markers p62/SQSTM1 (sequestosome 1), Beclin-1 and LC3B (microtubule associated protein 1 light chain 3 beta) were significantly increased together with the Iba-1 (ionized calcium binding adaptor molecule 1) mononuclear phagocyte marker and an enlargement of RPE size. These histopathological changes of RPE were accompanied by photoreceptor dysmorphology and vision loss as revealed by electroretinography. Consequently, these novel findings suggest that the NRF-2/PGC-1α dKO mouse is a valuable model for investigating the role of proteasomal and autophagy clearance in the RPE and in the development of dry AMD.

AB - Age-related macular degeneration (AMD) is a multi-factorial disease that is the leading cause of irreversible and severe vision loss in the developed countries. It has been suggested that the pathogenesis of dry AMD involves impaired protein degradation in retinal pigment epithelial cells (RPE). RPE cells are constantly exposed to oxidative stress that may lead to the accumulation of damaged cellular proteins, DNA and lipids and evoke tissue deterioration during the aging process. The ubiquitin-proteasome pathway and the lysosomal/autophagosomal pathway are the two major proteolytic systems in eukaryotic cells. NRF-2 (nuclear factor-erythroid 2-related factor-2) and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1 alpha) are master transcription factors in the regulation of cellular detoxification. We investigated the role of NRF-2 and PGC-1α in the regulation of RPE cell structure and function by using global double knockout (dKO) mice. The NRF-2/PGC-1α dKO mice exhibited significant age-dependent RPE degeneration, accumulation of the oxidative stress marker, 4-HNE (4-hydroxynonenal), the endoplasmic reticulum stress markers GRP78 (glucose-regulated protein 78) and ATF4 (activating transcription factor 4), and damaged mitochondria. Moreover, levels of protein ubiquitination and autophagy markers p62/SQSTM1 (sequestosome 1), Beclin-1 and LC3B (microtubule associated protein 1 light chain 3 beta) were significantly increased together with the Iba-1 (ionized calcium binding adaptor molecule 1) mononuclear phagocyte marker and an enlargement of RPE size. These histopathological changes of RPE were accompanied by photoreceptor dysmorphology and vision loss as revealed by electroretinography. Consequently, these novel findings suggest that the NRF-2/PGC-1α dKO mouse is a valuable model for investigating the role of proteasomal and autophagy clearance in the RPE and in the development of dry AMD.

KW - Aging

KW - Autophagy

KW - Degeneration

KW - Oxidative stress

KW - Proteasome

KW - Protein aggregation

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