Loss of GSNOR1 function leads to compromised auxin signaling and polar auxin transport

Ya Fei Shi, Da Li Wang, Chao Wang, Angela Hendrickson Culler, Molly A. Kreiser, Jayanti Suresh, Jerry D. Cohen, Jianwei Pan, Barbara Baker, Jian Zhong Liu

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38 Scopus citations

Abstract

Cross talk between phytohormones, nitric oxide (NO), and auxin has been implicated in the control of plant growth and development. Two recent reports indicate that NO promoted auxin signaling but inhibited auxin transport probably through S-nitrosylation. However, genetic evidence for the effect of S-nitrosylation on auxin physiology has been lacking. In this study, we used a genetic approach to understand the broader role of S-nitrosylation in auxin physiology in Arabidopsis. We compared auxin signaling and transport in Col-0 and gsnor1-3, a loss-of-function GSNOR1 mutant defective in protein de-nitrosylation. Our results showed that auxin signaling was impaired in the gsnor1-3 mutant as revealed by significantly reduced DR5-GUS/DR5-GFP accumulation and compromised degradation of AXR3NT-GUS, a useful reporter in interrogating auxin-mediated degradation of Aux/IAA by auxin receptors. In addition, polar auxin transport was compromised in gsnor1-3, which was correlated with universally reduced levels of PIN or GFP-PIN proteins in the roots of the mutant in a manner independent of transcription and 26S proteasome degradation. Our results suggest that S-nitrosylation and GSNOR1-mediated de-nitrosylation contribute to auxin physiology, and impaired auxin signaling and compromised auxin transport are responsible for the auxin-related morphological phenotypes displayed by the gsnor1-3 mutant.

Original languageEnglish (US)
Pages (from-to)1350-1365
Number of pages16
JournalMolecular Plant
Volume8
Issue number9
DOIs
StatePublished - Sep 7 2015

Bibliographical note

Funding Information:
This work was supported by the Natural Science Foundation of China ( 31371401 ); Qianjiang Talent Program of Zhejiang Province ( 2013R10074 ), the Natural Science Foundation of Zhejiang Province ( LY12C14001 ), and a special grant from Ministry of Human Resources and Social Security of China to scholars with research experience overseas ( ZC304013131 ) to J.Z.L.; the National Science Foundation of China ( 91317304 and 31370313 ) to J.W.P. and the U.S. National Science Foundation ( IOS-1238812 ) to J.D.C.

Keywords

  • Arabidopsis
  • S-nitrosoglutathione reductase (GSNOR)
  • S-nitrosylation
  • auxin signaling
  • auxin transport
  • phytohormone cross talk

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