Longitudinal study of [D10]phenanthrene metabolism by the diol epoxide pathway in smokers

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Abstract

The extent of metabolism of [D10]phenanthrene to [D 10]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetradeuterophenanthrene ([D10]PheT) could be a biomarker of human metabolic activation of carcinogenic polycyclic aromatic hydrocarbons, leading to identification of smokers particularly susceptible to lung cancer. The longitudinal stability of [D10]PheT was evaluated in 24 cigaret smokers given 7-8 oral doses of [D 10]phenanthrene (10°g) over 5.5 months. [D10]PheT in 6h urine was quantified after each dose. The overall coefficient of variation for 24 subjects was (mean±S.D.) 27.4%±8.83%. Thus, a single administration of [D10]phenanthrene is likely sufficient to determine a smoker's ability to metabolize it to [D10]PheT.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalBiomarkers
Volume18
Issue number2
DOIs
StatePublished - Mar 2013

Bibliographical note

Funding Information:
This study was supported by Grant No. CA-92025 from the U.S. National Cancer Institute. Mass spectrometry was carried out in the Analytical Biochemistry Shared Resource of the Masonic Cancer Center, supported in part by Cancer Center Support Grant CA-77498.

Keywords

  • Carcinogenic polycyclic aromatic hydrocarbons
  • Cigaret smokers
  • Urinary metabolites
  • [D10]phenanthrene
  • [D]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4- tetradeuterophenanthrene ([D]PheT)

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