Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation

Steven G Carmella, Alisa K. Heskin, Mei Kuen Tang, Joni Jensen, Xianghua Luo, Chap T Le, Sharon E Murphy, Neal L. Benowitz, F. Joseph McClernon, Ryan Vandrey, Sharon S Allen, Rachel Denlinger-Apte, Paul M. Cinciripini, Andrew A. Strasser, Mustafa N al'Absi, Jason D. Robinson, Eric C. Donny, Dorothy K Hatsukami, Stephen S Hecht

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Abstract

The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95% CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.

Original languageEnglish (US)
Article numbere0215853
JournalPloS one
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2019

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eicosanoids
Eicosanoids
cigarettes
Prostaglandins F
Biomarkers
Tobacco Products
Metabolites
biomarkers
prostaglandins
inflammation
Prostaglandins E
metabolites
Inflammation
creatinine
Creatinine
F2-Isoprostanes
smoking (habit)
nicotine
Electrospray ionization
smoke

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Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation. / Carmella, Steven G; Heskin, Alisa K.; Tang, Mei Kuen; Jensen, Joni; Luo, Xianghua; Le, Chap T; Murphy, Sharon E; Benowitz, Neal L.; Joseph McClernon, F.; Vandrey, Ryan; Allen, Sharon S; Denlinger-Apte, Rachel; Cinciripini, Paul M.; Strasser, Andrew A.; al'Absi, Mustafa N; Robinson, Jason D.; Donny, Eric C.; Hatsukami, Dorothy K; Hecht, Stephen S.

In: PloS one, Vol. 14, No. 4, e0215853, 01.04.2019.

Research output: Contribution to journalArticle

Carmella, SG, Heskin, AK, Tang, MK, Jensen, J, Luo, X, Le, CT, Murphy, SE, Benowitz, NL, Joseph McClernon, F, Vandrey, R, Allen, SS, Denlinger-Apte, R, Cinciripini, PM, Strasser, AA, al'Absi, MN, Robinson, JD, Donny, EC, Hatsukami, DK & Hecht, SS 2019, 'Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation', PloS one, vol. 14, no. 4, e0215853. https://doi.org/10.1371/journal.pone.0215853
Carmella, Steven G ; Heskin, Alisa K. ; Tang, Mei Kuen ; Jensen, Joni ; Luo, Xianghua ; Le, Chap T ; Murphy, Sharon E ; Benowitz, Neal L. ; Joseph McClernon, F. ; Vandrey, Ryan ; Allen, Sharon S ; Denlinger-Apte, Rachel ; Cinciripini, Paul M. ; Strasser, Andrew A. ; al'Absi, Mustafa N ; Robinson, Jason D. ; Donny, Eric C. ; Hatsukami, Dorothy K ; Hecht, Stephen S. / Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation. In: PloS one. 2019 ; Vol. 14, No. 4.
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abstract = "The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF 2α ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF 2α and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF 2α at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF 2α and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95{\%} CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF 2α and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF 2α and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF 2α and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.",
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T1 - Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation

AU - Carmella, Steven G

AU - Heskin, Alisa K.

AU - Tang, Mei Kuen

AU - Jensen, Joni

AU - Luo, Xianghua

AU - Le, Chap T

AU - Murphy, Sharon E

AU - Benowitz, Neal L.

AU - Joseph McClernon, F.

AU - Vandrey, Ryan

AU - Allen, Sharon S

AU - Denlinger-Apte, Rachel

AU - Cinciripini, Paul M.

AU - Strasser, Andrew A.

AU - al'Absi, Mustafa N

AU - Robinson, Jason D.

AU - Donny, Eric C.

AU - Hatsukami, Dorothy K

AU - Hecht, Stephen S

PY - 2019/4/1

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N2 - The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF 2α ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF 2α and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF 2α at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF 2α and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95% CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF 2α and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF 2α and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF 2α and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.

AB - The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF 2α ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF 2α and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF 2α at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF 2α and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95% CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF 2α and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF 2α and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF 2α and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.

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