Abstract
The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF 2α ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF 2α and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF 2α at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF 2α and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95% CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF 2α and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF 2α and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF 2α and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.
| Original language | English (US) |
|---|---|
| Article number | e0215853 |
| Journal | PloS one |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2019 |
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Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation. / Carmella, Steven G.; Heskin, Alisa K.; Tang, Mei Kuen; Jensen, Joni; Luo, Xianghua; Le, Chap T.; Murphy, Sharon E.; Benowitz, Neal L.; Joseph McClernon, F.; Vandrey, Ryan; Allen, Sharon S.; Denlinger-Apte, Rachel; Cinciripini, Paul M.; Strasser, Andrew A.; Al’Absi, Mustafa; Robinson, Jason D.; Donny, Eric C.; Hatsukami, Dorothy K.; Hecht, Stephen S.
In: PloS one, Vol. 14, No. 4, e0215853, 04.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Longitudinal stability in cigarette smokers of urinary eicosanoid biomarkers of oxidative damage and inflammation
AU - Carmella, Steven G.
AU - Heskin, Alisa K.
AU - Tang, Mei Kuen
AU - Jensen, Joni
AU - Luo, Xianghua
AU - Le, Chap T.
AU - Murphy, Sharon E.
AU - Benowitz, Neal L.
AU - Joseph McClernon, F.
AU - Vandrey, Ryan
AU - Allen, Sharon S.
AU - Denlinger-Apte, Rachel
AU - Cinciripini, Paul M.
AU - Strasser, Andrew A.
AU - Al’Absi, Mustafa
AU - Robinson, Jason D.
AU - Donny, Eric C.
AU - Hatsukami, Dorothy K.
AU - Hecht, Stephen S.
PY - 2019/4
Y1 - 2019/4
N2 - The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF 2α ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF 2α and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF 2α at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF 2α and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95% CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF 2α and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF 2α and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF 2α and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.
AB - The urinary metabolites (Z)-7-[1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl] cyclopentyl]hept-5-enoic acid (8-iso-PGF 2α ), an F2-isoprostane and biomarker of oxidative damage, and “prostaglandin E 2 metabolite” (PGE-M), a biomarker of inflammation, are elevated in cigarette smokers. However, there is little information in the literature on the longitudinal stability of these widely used biomarkers. In a large clinical trial involving 10 institutional sites, smokers were given, free of charge over a period of 20 weeks, Spectrum NRC600/601 research cigarettes containing 15.5 mg nicotine/g tobacco. All participants were instructed to smoke these cigarettes for the duration of the study. At weeks 4, 8, 12, 16, and 20, first morning urine voids were collected and analyzed for 8-iso-PGF 2α and PGE-M using validated liquid chromatography-electrospray ionization-tandem mass spectrometry methods. The mean level of 8-iso-PGF 2α at Week 4 was 1.34 ± 1.08 (S.D.) pmol/mg creatinine (N = 226) while that of PGE-M was 73.7 ± 113 (S.D.) pmol/mg creatinine (N = 232). The corresponding levels at Week 20 were 1.35 ± 0.93 (S.D.) pmol/mg creatinine (N = 209) for 8-iso-PGF 2α and 74.2 ± 142 (S.D.) pmol/mg creatinine (N = 210) for PGE-M. There was variation in these values in the intervening weeks. The intra-class correlation coefficients (ICC) were 0.51 (95% CI, 0.45, 0.57) and 0.36 (0.30, 0.43), for 8-iso-PGF 2α and PGE-M, respectively, indicating fair longitudinal stability for 8-iso-PGF 2α and poorer longitudinal stability for PGE-M in cigarette smokers. Males had higher ICC values than females for both 8-iso-PGF 2α and PGE-M. These results indicate that, in addition to cigarette smoking, endogenous processes of oxidative damage and inflammation influence the levels of these biomarkers over time among current smokers.
UR - http://www.scopus.com/inward/record.url?scp=85064867621&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064867621&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0215853
DO - 10.1371/journal.pone.0215853
M3 - Article
C2 - 31022220
AN - SCOPUS:85064867621
VL - 14
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 4
M1 - e0215853
ER -