TY - JOUR
T1 - Longitudinal assessment of two Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays in challenged and contact-exposed pigs
AU - Fano, Eduardo
AU - Pijoan, Carlos
AU - Dee, Scott
AU - Deen, John
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/3
Y1 - 2012/3
N2 - The objective of the current study was to compare the sensitivity of 2 Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays (ELISAs) in experimentally challenged and contact-exposed pigs in a long-term longitudinal assessment. On day 0 of the assessment, twelve 2-month-old M. hyopneumoniae-negative pigs were inoculated with M. hyopneumoniae strain 232 (group A). Twelve negative pigs were placed alongside the inoculated pigs, allowing direct contact exposure (group B). A third group of 12 pigs was allocated into 2 independent pens; no direct contact was allowed (group C). A longitudinal serologic profile was performed; samples were collected on days 0, 28, 35, 42, 49, 63, 91, 119, 154, and 170 of the study. Serum samples were tested using a blocking ELISA and an indirect ELISA. Results of the study demonstrated higher sensitivity of the blocking ELISA during early infection (clinical signs). Both ELISAs were 100% sensitive in challenged and naturally infected groups at several testing points during late infection (63, 91, 119, 154, and 170 days of the study) and showed a long antibody detection period. Both tests worked equally well during the chronic phase of infection but the blocking ELISA was more sensitive during acute stages of infection.
AB - The objective of the current study was to compare the sensitivity of 2 Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays (ELISAs) in experimentally challenged and contact-exposed pigs in a long-term longitudinal assessment. On day 0 of the assessment, twelve 2-month-old M. hyopneumoniae-negative pigs were inoculated with M. hyopneumoniae strain 232 (group A). Twelve negative pigs were placed alongside the inoculated pigs, allowing direct contact exposure (group B). A third group of 12 pigs was allocated into 2 independent pens; no direct contact was allowed (group C). A longitudinal serologic profile was performed; samples were collected on days 0, 28, 35, 42, 49, 63, 91, 119, 154, and 170 of the study. Serum samples were tested using a blocking ELISA and an indirect ELISA. Results of the study demonstrated higher sensitivity of the blocking ELISA during early infection (clinical signs). Both ELISAs were 100% sensitive in challenged and naturally infected groups at several testing points during late infection (63, 91, 119, 154, and 170 days of the study) and showed a long antibody detection period. Both tests worked equally well during the chronic phase of infection but the blocking ELISA was more sensitive during acute stages of infection.
KW - Enzyme-linked immunosorbent assay
KW - Mycoplasma hyopneumoniae
KW - pigs
KW - serology
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U2 - 10.1177/1040638711434942
DO - 10.1177/1040638711434942
M3 - Article
C2 - 22379055
AN - SCOPUS:84857761059
VL - 24
SP - 383
EP - 387
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 2
ER -