Longitudinal assessment of two Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays in challenged and contact-exposed pigs

Eduardo Fano, Carlos Pijoan, Scott Dee, John Deen

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The objective of the current study was to compare the sensitivity of 2 Mycoplasma hyopneumoniae enzyme-linked immunosorbent assays (ELISAs) in experimentally challenged and contact-exposed pigs in a long-term longitudinal assessment. On day 0 of the assessment, twelve 2-month-old M. hyopneumoniae-negative pigs were inoculated with M. hyopneumoniae strain 232 (group A). Twelve negative pigs were placed alongside the inoculated pigs, allowing direct contact exposure (group B). A third group of 12 pigs was allocated into 2 independent pens; no direct contact was allowed (group C). A longitudinal serologic profile was performed; samples were collected on days 0, 28, 35, 42, 49, 63, 91, 119, 154, and 170 of the study. Serum samples were tested using a blocking ELISA and an indirect ELISA. Results of the study demonstrated higher sensitivity of the blocking ELISA during early infection (clinical signs). Both ELISAs were 100% sensitive in challenged and naturally infected groups at several testing points during late infection (63, 91, 119, 154, and 170 days of the study) and showed a long antibody detection period. Both tests worked equally well during the chronic phase of infection but the blocking ELISA was more sensitive during acute stages of infection.

Original languageEnglish (US)
Pages (from-to)383-387
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number2
DOIs
StatePublished - Mar 2012

Keywords

  • Enzyme-linked immunosorbent assay
  • Mycoplasma hyopneumoniae
  • pigs
  • serology

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