Long-term vascular contractility assay using genipin-modified muscular thin films

Eric S. Hald, Kerianne E. Steucke, Jack A. Reeves, Zaw Win, Patrick W. Alford

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Vascular disease is a leading cause of death globally and typically manifests chronically due to long-term maladaptive arterial growth and remodeling. To date, there is no in vitro technique for studying vascular function over relevant disease time courses that both mimics in vivo-like tissue structure and provides a simple readout of tissue stress. We aimed to extend tissue viability in our muscular thin film contractility assay by modifying the polydimethylsiloxane (PDMS) substrate with micropatterned genipin, allowing extracellular matrix turnover without cell loss. To achieve this, we developed a microfluidic delivery system to pattern genipin and extracellular matrix proteins on PDMS prior to cell seeding. Tissues constructed using this method showed improved viability and maintenance of in vivo-like lamellar structure. Functional contractility of tissues fabricated on genipin-modified substrates remained consistent throughout two weeks in culture. These results suggest that muscular thin films with genipin-modified PDMS substrates are a viable method for conducting functional studies of arterial growth and remodeling in vascular diseases.

Original languageEnglish (US)
Article number045005
JournalBiofabrication
Volume6
Issue number4
DOIs
StatePublished - Dec 1 2014

Keywords

  • arterial tissue engineering
  • cell viability
  • in vitro disease model
  • mechanical properties
  • polydimethylsiloxane
  • vascular smooth muscle cells

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