Using cultured endothelial cells, the present study investigates the effect of the molsidomine metabolite and nitric oxide donor linsidomine (SIN-1) on hydrogen peroxide-induced cytotoxicity and expression of ferritin heavy-chain mRNA. In porcine aortic endothelial cells, a 20-h incubation with hydrogen peroxide (0.5 mM) reduced the number of viable cells to 44% of control. A 6-h preincubation with SIN-1 (0.5 mM) protected endothelial cells from hydrogen peroxide-dependent cytotoxicity and increased viability to 81% of control. This protection was associated with a 2-fold induction of ferritin heavy-chain mRNA by SIN-1. A protective effect comparable with SIN-1 was observed when preincubating the cells with iron-free apoferritin (1 mg mL-1). These findings suggest that ferritin induction may be a mechanism underlying long-term cytoprotection by SIN-1 against oxidative stress.
|Original language||English (US)|
|Number of pages||3|
|State||Published - Dec 1 1996|