Long-term maintenance of a transgenic Catharanthus roseus hairy root line

Christie A.M. Peebles, Sue Gibson, Jacqueline V. Shanks, Ka Yiu San

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.

Original languageEnglish (US)
Pages (from-to)1517-1518
Number of pages2
JournalBiotechnology Progress
Volume23
Issue number6
DOIs
StatePublished - Nov 1 2007

Fingerprint

Catharanthus
Catharanthus roseus
Green Fluorescent Proteins
Transgenes
Dexamethasone
Glucocorticoids
genetically modified organisms
Polymerase Chain Reaction
dexamethasone
glucocorticoids
green fluorescent protein
secondary metabolites
transgenes
promoter regions
genomics

Cite this

Long-term maintenance of a transgenic Catharanthus roseus hairy root line. / Peebles, Christie A.M.; Gibson, Sue; Shanks, Jacqueline V.; San, Ka Yiu.

In: Biotechnology Progress, Vol. 23, No. 6, 01.11.2007, p. 1517-1518.

Research output: Contribution to journalArticle

Peebles, Christie A.M. ; Gibson, Sue ; Shanks, Jacqueline V. ; San, Ka Yiu. / Long-term maintenance of a transgenic Catharanthus roseus hairy root line. In: Biotechnology Progress. 2007 ; Vol. 23, No. 6. pp. 1517-1518.
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