Long-lived and short-lived reactive species produced by a cold atmospheric pressure plasma jet for the inactivation of Pseudomonas aeruginosa and Staphylococcus aureus

V. S.Santosh K. Kondeti, Chi Q. Phan, Kristian Wende, Helena Jablonowski, Urvashi Gangal, Jennifer L. Granick, Ryan C. Hunter, Peter J. Bruggeman

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Different chemical pathways leading to the inactivation of Pseudomonas aeruginosa and Staphylococcus aureus by a cold atmospheric pressure plasma jet (APPJ) in buffered and non-buffered solutions are reported. As APPJs produce a complex mixture of reactive species in solution, a comprehensive set of diagnostics were used to assess the liquid phase chemistry. This includes absorption and electron paramagnetic resonance spectroscopy in addition to a scavenger study to assess the relative importance of the various plasma produced species involved in the inactivation of bacteria. Different modes of inactivation of bacteria were found for the same plasma source depending on the solution and the plasma feed gas. The inactivation of bacteria in saline is due to the production of short-lived species in the case of argon plasma when the plasma touches the liquid. Long-lived species (ClO ) formed by the abundant amount of O. radicals produced by the plasmas played a dominant role in the case of Ar + 1% O 2 and Ar + 1% air plasmas when the plasma is not in direct contact with the liquid. Inactivation of bacteria in distilled water was found to be due to the generation of short-lived species: O. & O 2 .− for Ar + 1% O 2 plasma and O 2 .− (and .OH in absence of saline) for Ar plasma.

Original languageEnglish (US)
Pages (from-to)275-287
Number of pages13
JournalFree Radical Biology and Medicine
Volume124
DOIs
StatePublished - Aug 20 2018

Bibliographical note

Funding Information:
This work is funded by the Department of Energy Plasma Science Center through the US Department of Energy, Office of Fusion Energy Sciences , Contract: DE-SC0001939 , the University of Minnesota, the German Federal Ministry of Education and Research (BMBF) (Grant No. 03Z22DN12 ) and a Signature Medicine grant from the University of Minnesota. The authors would like to thank Hamed Taghvaei for the preparation of TA solutions.

Publisher Copyright:
© 2018

Keywords

  • .OH
  • Atmospheric pressure plasma
  • Bacteria inactivation
  • ClO
  • Electron spin resonance
  • O
  • O.
  • Plasma medicine
  • Pseudomonas aeruginosa
  • Staphylococcus aureus

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