Localized Genetic and Phenotypic Diversity of Xanthomonas translucens Associated with Bacterial Leaf Streak on Wheat and Barley in Minnesota

Rebecca D. Curland, Liangliang Gao, Cory D. Hirsch, Carol A. Ishimaru

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial leaf streak (BLS) of wheat and barley has been a disease of increasing concern in the Upper Midwest over the past decade. In this study, intra- and interfield genetic and pathogenic diversity of bacteria causing BLS in Minnesota was evaluated. In 2015, 89 strains were isolated from 100 leaf samples collected from two wheat and two barley fields naturally infected with BLS. Virulence assays and multilocus sequence alignments of four housekeeping genes supported pathovar identifications. All wheat strains were pathogenic on wheat and barley and belonged to the same lineage as the Xanthomonas translucens pv. undulosa-type strain. All barley strains were pathogenic on barley but not on wheat. Three lineages of barley strains were detected. The frequency and number of sequence types of each pathovar varied within and between fields. A significant population variance was detected between populations of X. translucens pv. undulosa collected from different wheat fields. Population stratification of X. translucens pv. translucens was not detected. Significant differences in virulence were detected among three dominant sequence types of X. translucens pv. undulosa but not those of X. translucens pv. translucens. Field trials with wheat and barley plants inoculated with strains of known sequence type and virulence did not detect significant race structures within either pathovar. Knowledge of virulence, sequence types, and population structures of X. translucens on wheat and barley can support studies on plant–bacterial interactions and breeding for BLS disease resistance.

Original languageEnglish (US)
Pages (from-to)257-266
Number of pages10
JournalPhytopathology
Volume110
Issue number2
DOIs
StatePublished - 2020

Bibliographical note

Funding Information:
Funding: Funding for this project was provided by the Minnesota Wheat Research and Promotion Council and the Minnesota Small Grains Initiative.

Publisher Copyright:
© 2020 The American Phytopathological Society

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • Bacterial populations
  • Bacteriology
  • Ecology and epidemiology
  • Multilocus sequence alignment
  • Multilocus sequence typing
  • Population biology

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Localized Genetic and Phenotypic Diversity of Xanthomonas translucens Associated with Bacterial Leaf Streak on Wheat and Barley in Minnesota'. Together they form a unique fingerprint.

Cite this