Localization of vasoactive intestinal peptide binding sites in the thymus and bursa of Fabricius of the chick

Carolyn B. Lacey, Robert P. Elde, Virginia S. Seybold

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The purpose of this study was to determine the distribution of VIP binding sites in the thymus and bursa of Fabricius using receptor binding and autoradiographic techniques. Biochemical characterization of 125I-VIP binding sites determined two classes of specific binding sites in both tissues. The dissociation constants determined in the thymus were 1.12 nM and 88.5 nM, and in the bursa were 0.459 nM and 70.8 nM. Autoradiographic localization of 125I-VIP binding sites within the thymus demonstrated specific binding associated with the medullary region of the thymic lobule and the blood vessels in the interlobular and trabecular areas. Within the bursa of Fabricius, high densities of silver grains corresponded with vascular elements in the interfollicular regions, the epithelial border of the plicae, the muscular layer surrounding the organ, and the diffusely infiltrated area near the burso-cloacal duct.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalPeptides
Volume12
Issue number2
DOIs
StatePublished - 1991

Bibliographical note

Funding Information:
We thank Lia M. Haynes for technical assistance and Jerry Sedgewick for photographic assistance. Studies were funded by DA02148, the Graduate School of the University of Minnesota, the Minnesota Medical Foundation, and the 3M Foundation.

Keywords

  • Autoradiography
  • Bursa of Fabricius
  • Neuroimmune interaction
  • Thymus
  • Vasoactive intestinal peptide (VIP)

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