Localization of the metJBLF gene cluster of Escherichia coli in λmet transducing phage

Ronald C. Greene, Judy H. Krueger, James R. Johnson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The position of the metJBLF gene cluster in the transducing phage λd met102 was determined by ligation of its leftmost EcoRI fragment (102-1) to the λBCDEF (nin5) EcoRI fragment of λgtl (λBC) and characterization of the resultant recombinant phage. The new transducing phage carries about 6kb of bacterial DNA which contains the entire met gene cluster including the promoter of its rightmost member metF. Reasonable estimates of the coding capacity required for the four genes indicate that most of the bacterial DNA of the recombinant phage is occupied by the met gene cluster.

Original languageEnglish (US)
Pages (from-to)401-404
Number of pages4
JournalMGG Molecular & General Genetics
Volume187
Issue number3
DOIs
StatePublished - Oct 1 1982

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