A panel of antibodies was produced using ruffe sperm and testicular tissue as the immunogen. Eight hybridoma cell lines were selected by screening formaldehyde-fixed cryostat sections of testes. Based on immunolabeling patterns, five groups of testicular antigens were identified: 1) 'sperm surface' (entire surface of sperm labeled), 2) 'punctate' (a few loci on each sperm head labeled), 3) 'painted' (filamentous material within the spermatocyst labeled), 4) 'lumenal' (particulate material within the spermatocyst labeled), and 5) 'CT' (testicular connective tissue is labeled). The hybridomas that produced antibodies recognizing antigens located on the surface of ruffe sperm are of particular interest because of the possibility that they may be identifying molecules involved in sperm-egg binding and fertilization.
Bibliographical noteFunding Information:
This work is the result of research sponsored by the Minnesota Sea Grant College Program supported by the NOAA Office of Sea Grant, United States Department of Commerce, under grant No. NA46RG0101. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 437 of the Minnesota Sea Grant College Program.
- Monoclonal antibodies