Localization of ruffe testicular antigens by a panel of antibodies

Kevin Flynn; Patrick K Schoff; Jon M Holy

doi:10.1016/S0380-1330(98)70828-7

Localization of ruffe testicular antigens by a panel of antibodies

Kevin Flynn, Patrick K Schoff, Jon M Holy

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A panel of antibodies was produced using ruffe sperm and testicular tissue as the immunogen. Eight hybridoma cell lines were selected by screening formaldehyde-fixed cryostat sections of testes. Based on immunolabeling patterns, five groups of testicular antigens were identified: 1) 'sperm surface' (entire surface of sperm labeled), 2) 'punctate' (a few loci on each sperm head labeled), 3) 'painted' (filamentous material within the spermatocyst labeled), 4) 'lumenal' (particulate material within the spermatocyst labeled), and 5) 'CT' (testicular connective tissue is labeled). The hybridomas that produced antibodies recognizing antigens located on the surface of ruffe sperm are of particular interest because of the possibility that they may be identifying molecules involved in sperm-egg binding and fertilization.

Original language	English (US)
Pages (from-to)	379-382
Number of pages	4
Journal	Journal of Great Lakes Research
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/S0380-1330(98)70828-7
State	Published - 1998

Bibliographical note

Funding Information:
This work is the result of research sponsored by the Minnesota Sea Grant College Program supported by the NOAA Office of Sea Grant, United States Department of Commerce, under grant No. NA46RG0101. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 437 of the Minnesota Sea Grant College Program.

Keywords

Immunocytochemistry
Monoclonal antibodies
Ruffe
Sperm
Testis

Publisher link

10.1016/S0380-1330(98)70828-7

Find It

Find It at U of M Libraries

Cite this

@article{b84f191ffe404793bacfc7e45241df9f,

title = "Localization of ruffe testicular antigens by a panel of antibodies",

abstract = "A panel of antibodies was produced using ruffe sperm and testicular tissue as the immunogen. Eight hybridoma cell lines were selected by screening formaldehyde-fixed cryostat sections of testes. Based on immunolabeling patterns, five groups of testicular antigens were identified: 1) 'sperm surface' (entire surface of sperm labeled), 2) 'punctate' (a few loci on each sperm head labeled), 3) 'painted' (filamentous material within the spermatocyst labeled), 4) 'lumenal' (particulate material within the spermatocyst labeled), and 5) 'CT' (testicular connective tissue is labeled). The hybridomas that produced antibodies recognizing antigens located on the surface of ruffe sperm are of particular interest because of the possibility that they may be identifying molecules involved in sperm-egg binding and fertilization.",

keywords = "Immunocytochemistry, Monoclonal antibodies, Ruffe, Sperm, Testis",

author = "Kevin Flynn and Schoff, {Patrick K} and Holy, {Jon M}",

note = "Funding Information: This work is the result of research sponsored by the Minnesota Sea Grant College Program supported by the NOAA Office of Sea Grant, United States Department of Commerce, under grant No. NA46RG0101. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 437 of the Minnesota Sea Grant College Program.",

year = "1998",

doi = "10.1016/S0380-1330(98)70828-7",

language = "English (US)",

volume = "24",

pages = "379--382",

journal = "Journal of Great Lakes Research",

issn = "0380-1330",

publisher = "International Association of Great Lakes Research",

number = "2",

}

TY - JOUR

T1 - Localization of ruffe testicular antigens by a panel of antibodies

AU - Flynn, Kevin

AU - Schoff, Patrick K

AU - Holy, Jon M

N1 - Funding Information: This work is the result of research sponsored by the Minnesota Sea Grant College Program supported by the NOAA Office of Sea Grant, United States Department of Commerce, under grant No. NA46RG0101. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 437 of the Minnesota Sea Grant College Program.

PY - 1998

Y1 - 1998

N2 - A panel of antibodies was produced using ruffe sperm and testicular tissue as the immunogen. Eight hybridoma cell lines were selected by screening formaldehyde-fixed cryostat sections of testes. Based on immunolabeling patterns, five groups of testicular antigens were identified: 1) 'sperm surface' (entire surface of sperm labeled), 2) 'punctate' (a few loci on each sperm head labeled), 3) 'painted' (filamentous material within the spermatocyst labeled), 4) 'lumenal' (particulate material within the spermatocyst labeled), and 5) 'CT' (testicular connective tissue is labeled). The hybridomas that produced antibodies recognizing antigens located on the surface of ruffe sperm are of particular interest because of the possibility that they may be identifying molecules involved in sperm-egg binding and fertilization.

AB - A panel of antibodies was produced using ruffe sperm and testicular tissue as the immunogen. Eight hybridoma cell lines were selected by screening formaldehyde-fixed cryostat sections of testes. Based on immunolabeling patterns, five groups of testicular antigens were identified: 1) 'sperm surface' (entire surface of sperm labeled), 2) 'punctate' (a few loci on each sperm head labeled), 3) 'painted' (filamentous material within the spermatocyst labeled), 4) 'lumenal' (particulate material within the spermatocyst labeled), and 5) 'CT' (testicular connective tissue is labeled). The hybridomas that produced antibodies recognizing antigens located on the surface of ruffe sperm are of particular interest because of the possibility that they may be identifying molecules involved in sperm-egg binding and fertilization.

KW - Immunocytochemistry

KW - Monoclonal antibodies

KW - Ruffe

KW - Sperm

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0031853724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031853724&partnerID=8YFLogxK

U2 - 10.1016/S0380-1330(98)70828-7

DO - 10.1016/S0380-1330(98)70828-7

M3 - Article

AN - SCOPUS:0031853724

SN - 0380-1330

VL - 24

SP - 379

EP - 382

JO - Journal of Great Lakes Research

JF - Journal of Great Lakes Research

IS - 2

ER -

Localization of ruffe testicular antigens by a panel of antibodies

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this