Localization of protein Ser/Thr phosphatase 5 in rat brain

Rimple Bahl; Katherine C. Bradley; Kenira J. Thompson; Rodney A. Swain; Sandra Rossie; Robert L. Meisel

doi:10.1016/S0169-328X(01)00089-4

Localization of protein Ser/Thr phosphatase 5 in rat brain

Rimple Bahl
, Katherine C. Bradley
, Kenira J. Thompson
, Rodney A. Swain
, Sandra Rossie
, Robert L. Meisel

Neuroscience

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Protein phosphatase 5 is a recently discovered Ser/Thr phosphatase that is structurally related to calcineurin and protein phosphatases 1 and 2. Northern blot and in situ hybridization studies have shown that protein phosphatase 5 mRNA is present at high levels in brain and is localized to discrete regions. In the present study, we used immunocytochemistry and immunoblot analyses to examine the regional and subcellular distribution of this enzyme in brain. Our work demonstrates that protein phosphatase 5 is widely expressed throughout brain, but is not uniformly distributed. The most intense staining occurred in neurons of the cerebellum, cerebral cortex, and the supraoptic nucleus of the hypothalamus. Other areas also contained immunoreactive cell bodies, including the globus pallidus, hippocampus, thalamus, lateral preoptic area of the hypothalamus, substantia nigra and other brainstem nuclei. Staining in these cells was observed primarily in perikarya and proximal processes.

Original language	English (US)
Pages (from-to)	101-109
Number of pages	9
Journal	Molecular Brain Research
Volume	90
Issue number	2
DOIs	https://doi.org/10.1016/S0169-328X(01)00089-4
State	Published - Jun 20 2001

Bibliographical note

Funding Information:
We thank Arthi Kanthasamy (Department of Molecular Pharmacology and Medicinal Chemistry, Purdue University) for help dissecting specific rat brain regions and Dr Robert J. Phillips (Department of Psychological Sciences, Purdue University) for guidance in image processing. This is Journal Paper 16415 from the Purdue University Agricultural Experimental Station. This work was supported by NIH grant NS31221 (SR) and NSF grant IBN-9723876 (RLM).

Keywords

Brain localization
Dephosphorylation
PP5
Protein phosphatase 5

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title = "Localization of protein Ser/Thr phosphatase 5 in rat brain",

abstract = "Protein phosphatase 5 is a recently discovered Ser/Thr phosphatase that is structurally related to calcineurin and protein phosphatases 1 and 2. Northern blot and in situ hybridization studies have shown that protein phosphatase 5 mRNA is present at high levels in brain and is localized to discrete regions. In the present study, we used immunocytochemistry and immunoblot analyses to examine the regional and subcellular distribution of this enzyme in brain. Our work demonstrates that protein phosphatase 5 is widely expressed throughout brain, but is not uniformly distributed. The most intense staining occurred in neurons of the cerebellum, cerebral cortex, and the supraoptic nucleus of the hypothalamus. Other areas also contained immunoreactive cell bodies, including the globus pallidus, hippocampus, thalamus, lateral preoptic area of the hypothalamus, substantia nigra and other brainstem nuclei. Staining in these cells was observed primarily in perikarya and proximal processes.",

keywords = "Brain localization, Dephosphorylation, PP5, Protein phosphatase 5",

author = "Rimple Bahl and Bradley, \{Katherine C.\} and Thompson, \{Kenira J.\} and Swain, \{Rodney A.\} and Sandra Rossie and Meisel, \{Robert L.\}",

note = "Funding Information: We thank Arthi Kanthasamy (Department of Molecular Pharmacology and Medicinal Chemistry, Purdue University) for help dissecting specific rat brain regions and Dr Robert J. Phillips (Department of Psychological Sciences, Purdue University) for guidance in image processing. This is Journal Paper 16415 from the Purdue University Agricultural Experimental Station. This work was supported by NIH grant NS31221 (SR) and NSF grant IBN-9723876 (RLM).",

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AU - Swain, Rodney A.

AU - Rossie, Sandra

AU - Meisel, Robert L.

N1 - Funding Information: We thank Arthi Kanthasamy (Department of Molecular Pharmacology and Medicinal Chemistry, Purdue University) for help dissecting specific rat brain regions and Dr Robert J. Phillips (Department of Psychological Sciences, Purdue University) for guidance in image processing. This is Journal Paper 16415 from the Purdue University Agricultural Experimental Station. This work was supported by NIH grant NS31221 (SR) and NSF grant IBN-9723876 (RLM).

PY - 2001/6/20

Y1 - 2001/6/20

N2 - Protein phosphatase 5 is a recently discovered Ser/Thr phosphatase that is structurally related to calcineurin and protein phosphatases 1 and 2. Northern blot and in situ hybridization studies have shown that protein phosphatase 5 mRNA is present at high levels in brain and is localized to discrete regions. In the present study, we used immunocytochemistry and immunoblot analyses to examine the regional and subcellular distribution of this enzyme in brain. Our work demonstrates that protein phosphatase 5 is widely expressed throughout brain, but is not uniformly distributed. The most intense staining occurred in neurons of the cerebellum, cerebral cortex, and the supraoptic nucleus of the hypothalamus. Other areas also contained immunoreactive cell bodies, including the globus pallidus, hippocampus, thalamus, lateral preoptic area of the hypothalamus, substantia nigra and other brainstem nuclei. Staining in these cells was observed primarily in perikarya and proximal processes.

AB - Protein phosphatase 5 is a recently discovered Ser/Thr phosphatase that is structurally related to calcineurin and protein phosphatases 1 and 2. Northern blot and in situ hybridization studies have shown that protein phosphatase 5 mRNA is present at high levels in brain and is localized to discrete regions. In the present study, we used immunocytochemistry and immunoblot analyses to examine the regional and subcellular distribution of this enzyme in brain. Our work demonstrates that protein phosphatase 5 is widely expressed throughout brain, but is not uniformly distributed. The most intense staining occurred in neurons of the cerebellum, cerebral cortex, and the supraoptic nucleus of the hypothalamus. Other areas also contained immunoreactive cell bodies, including the globus pallidus, hippocampus, thalamus, lateral preoptic area of the hypothalamus, substantia nigra and other brainstem nuclei. Staining in these cells was observed primarily in perikarya and proximal processes.

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KW - Dephosphorylation

KW - PP5

KW - Protein phosphatase 5

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Localization of protein Ser/Thr phosphatase 5 in rat brain

Abstract

Bibliographical note

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