Localization of prostaglandin endoperoxide synthase in the human corpus luteum

S. W. Kauma, T. E. Curry, D. E. Powell, M. R. Clark

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Prostaglandins have been implicated in both maintenance and luteolysis of the primate corpus luteum. Central to the production of prostaglandins is the enzyme prostaglandin endoperoxide synthase (PGHS). In the present study, we identified the cell types which contain PGHS in 44 human corpora lutea, using immunoperoxidase staining techniques. Intense granular staining was present in the cytoplasm of granulosa lutein cells of tissues obtained from the mid-luteal phase. Theca lutein cells demonstrated a diffuse cytoplasmic staining which was less intense than that observed in granulosa lutein cells. Staining appeared less intense in tisues from the early or late phase. Ovarian stromal cells demonstrated little or no PGHS immunoreactivity. PGHS staining in the corpus luteum of pregnancy was similar in intensity and cell distribution to that of mid-luted corpus luteum. In summary, human corpus luteum contains immunoreactive PGHS which localized mainly to welldifferentiated granulosa lutein cells.

Original languageEnglish (US)
Pages (from-to)800-804
Number of pages5
JournalHuman Reproduction
Issue number7
StatePublished - Oct 1990

Bibliographical note

Funding Information:
Tissue specimens were obtained with the assistance of F.B.Askin, M.D., Department of Pathology, University of North Carolina at Chapel Hill. The technical assistance of Carole Bryant in preparing the luteal sections is gratefully appreciated. The research was supported by NIH grants HD-22299, HD-18968, and the Department of Obstetrics and Gynecology.


  • Corpus luteum
  • Cyclooxygenase
  • Immunohistochemistry
  • Prostaglandin endoperoxide synthase


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