Localization of NADPH diaphorase in neurons of the rostral ventral medulla: possible role of nitric oxide in central autonomic regulation and oxygen chemoreception

Costantino Iadecola, Patricia L Faris, Boyd K Hartman, Xiaohong Xu

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102 Scopus citations

Abstract

We studied whether neurons containing nitric oxide synthase (NOS) are localized to the rostral ventrolateral medulla (RVM) and, if so, whether they are distinct from the adrenergic neurons of the C1 group. NOS-containing neurons and/or C1 neurons were visualized using NADPH diaphorase histochemistry and phenylethanolamine N-methyltransferase (PNMT) immunohistochemistry, respectively. A column of NADPH diaphorase-positive neurons, extending 2 mm in the rostrocaudal plane, was observed lateral to the inferior olive and medial to the C1 neurons. Double labelling studies showed that NADPH diaphorase-positive neurons were not immunoreactive for PNMT, indicating that the two enzymes were localized in the different cells. Furthermore, only a small fraction of NADPH diaphorase neurons were retrogradely labelled after injections of fluorogold into the thoracic cord. We conclude that the RVM contains a well-defined group of neurons endowed with NOS that are distinct from the adrenergic neurons of the C1 group and have only limited monosynaptic projections to the spinal cord. Since the RVM is involved in the control of arterial pressure and in oxygen-conserving reflexes, the findings raise the possibility that nitric oxide participates in central autonomic regulation and oxygen chemoreception.

Original languageEnglish (US)
Pages (from-to)173-179
Number of pages7
JournalBrain Research
Volume603
Issue number1
DOIs
StatePublished - Feb 12 1993

Bibliographical note

Funding Information:
This work was supported by Grants-in-Aids from the American Heart Association (Minnesota) and by NIH Grants MH47189 and NS12311.

Keywords

  • Double-labelling
  • Intermediolateral column
  • Nitric oxide synthase
  • Phenylethanolamine N-methyltransferase
  • Ventral medullary surface

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