Mass cultivation of microalgae for biofuel production depends heavily on the performance of the microalgae strains used. In this study, 60 algae-like microorganisms collected from different sampling sites in Minnesota were examined using multi-step screening and acclimation procedures to select high-lipid producing facultative heterotrophic microalgae strains capable of growing on concentrated municipal wastewater (CMW) for simultaneous energy crop production and wastewater treatment. Twenty-seven facultative heterotrophic microalgae strains were found, among which 17 strains were proved to be tolerant to CMW. These 17 top-performing strains were identified through morphological observation and DNA sequencing as Chlorella sp., Heynigia sp., Hindakia sp., Micractinium sp., and Scenedesmus sp. Five strains were chosen for other studies because of their ability to adapt to CMW, high growth rates (0.455-0.498d-1) and higher lipid productivities (74.5-77.8mgL-1d-1). These strains are considered highly promising compared with other strains reported in the literature.
Bibliographical noteFunding Information:
The study was supported by grants from the University of Minnesota Initiative for Renewable Energy and the Environment (IREE) , Metropolitan Council Environmental Services (MCES) , Xcel Energy , the Legislative-Citizen Commission on Minnesota Resources (LCCMR) . The authors are also grateful to Blanca C. Martinez for providing help in the labs and Adam Sealock from Saint Paul MCES Wastewater Treatment Plant for helping with the sample collection.
Copyright 2011 Elsevier B.V., All rights reserved.
- Biofuel production
- Heterotrophic microalgae
- Municipal wastewater