TY - JOUR
T1 - Liver histological, portal flow and plasmatic nitric oxide alterations caused by biliary obstruction and drainage in rats
AU - Dias, Miguel Angel
AU - Ceneviva, Reginaldo
AU - Elias, Jorge
AU - Zucoloto, Sergio
AU - Baldo, Caroline Floreoto
AU - Évora, Paulo Roberto Barbosa
PY - 2008
Y1 - 2008
N2 - Purpose: To evaluate liver alterations caused by biliary obstruction and drainage. Methods: Thirty-nine male Wistar rats were randomly distributed in 4 groups: BO (n=18) bile duct ligation for 20 days, with a periodic evaluation of liver histological alterations, Doppler echography portal flow and measurements of NO and malondialdehyde (MDA); BO/DB (n=13) bile duct occlusion for 20 days followed by biliary drainage by choledochoduodenal anastomosis, 5 days followup, same BO group parameters evaluations; group CED (n=4) sham operation and portal flow evaluation trough 20 days; CHB (n=4) sham operation, with hepatic biopsy on 25th day and followed-up trough 25 days, by the same parameters of group BO, with exception of portal flow. Direct bilirubin (DB) and alkaline phosphatase (AP) were evaluated in the group BO, BO/DB and CHB. Results: The bile duct ligation led to an increase of DB and AP, development of liver histological alterations, reduction of portal flow and increase of plasmatic NO and of MDA levels. The bile duct clearing resulted in a reduction of DB, AP, NO, MDA histological alterations and increase of portal flow. Conclusion: The biliary occlusion resulted in cholestasis and portal flow reduction, besides the increase of plasmatic NO and of hepatic MDA levels, and histological liver alterations, with a tendency of normalization after the bile duct clearing.
AB - Purpose: To evaluate liver alterations caused by biliary obstruction and drainage. Methods: Thirty-nine male Wistar rats were randomly distributed in 4 groups: BO (n=18) bile duct ligation for 20 days, with a periodic evaluation of liver histological alterations, Doppler echography portal flow and measurements of NO and malondialdehyde (MDA); BO/DB (n=13) bile duct occlusion for 20 days followed by biliary drainage by choledochoduodenal anastomosis, 5 days followup, same BO group parameters evaluations; group CED (n=4) sham operation and portal flow evaluation trough 20 days; CHB (n=4) sham operation, with hepatic biopsy on 25th day and followed-up trough 25 days, by the same parameters of group BO, with exception of portal flow. Direct bilirubin (DB) and alkaline phosphatase (AP) were evaluated in the group BO, BO/DB and CHB. Results: The bile duct ligation led to an increase of DB and AP, development of liver histological alterations, reduction of portal flow and increase of plasmatic NO and of MDA levels. The bile duct clearing resulted in a reduction of DB, AP, NO, MDA histological alterations and increase of portal flow. Conclusion: The biliary occlusion resulted in cholestasis and portal flow reduction, besides the increase of plasmatic NO and of hepatic MDA levels, and histological liver alterations, with a tendency of normalization after the bile duct clearing.
KW - Biliary obstruction
KW - Cholestasis
KW - Lipid peroxidation
KW - Liver
KW - Malondialdehyde
KW - Nitric oxide
KW - Portal flow
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U2 - 10.1590/S0102-86502008000700002
DO - 10.1590/S0102-86502008000700002
M3 - Article
C2 - 18516441
AN - SCOPUS:44449135525
SN - 0102-8650
VL - 23
SP - 2
EP - 7
JO - Acta Cirurgica Brasileira
JF - Acta Cirurgica Brasileira
IS - SUPPL.
ER -