Liver glycogen synthase and phosphorylase changes in vivo with hypoxia and anesthetics

J. Theen, D. P. Gilboe, F. Q. Nuttall

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Methods for obtaining and processing rat liver for determination of glycogen phosphorylase a and synthase I activity were studied. An extremely rapid and profound increase in phosphorylase was induced by hypoxia. The effect on synthase I was slower and less striking. Using α- and β-adrenergic antagonists, a catecholamine-depleting agent, and a ganglionic blocking agent, it was determined that adrenergic stimulation secondary to the surgical procedure required to obtain the liver was not a significant factor. The anesthetic agent used also had a significant effect on the proportion of phosphorylase in the a form. Seconal anesthesia resulted in lower phosphorylase a levels than did ether or urethan anesthesia.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume6
Issue number3
StatePublished - Sep 1982

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Phosphorylase a
Glycogen Phosphorylase
Glycogen Synthase
Phosphorylases
Liver Glycogen
Liver
Anesthetics
Secobarbital
Ganglionic Blockers
Adrenergic Antagonists
Urethane
Anesthesia
Ether
Adrenergic Agents
Catecholamines
Rats
Processing
Hypoxia

Cite this

Liver glycogen synthase and phosphorylase changes in vivo with hypoxia and anesthetics. / Theen, J.; Gilboe, D. P.; Nuttall, F. Q.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 6, No. 3, 09.1982.

Research output: Contribution to journalArticle

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