@inbook{921a5876f4b14ed79d26d96bc1dade61,
title = "Live imaging of LFA-1-dependent T-cell motility and stop signals",
abstract = "T-cell motility is critical for leukocyte trafficking both in normal host defense and in pathologic conditions including chronic inflammatory disease. Despite progress in understanding the mechanisms of T-cell polarity and motility, we have limited understanding of the mechanisms that contribute to antigen-induced T cell arrest. Here, we describe methods to analyze leukocyte function antigen-1-mediated T-cell motility and T-cell receptor-induced stop signals using in vitro assays on two-dimensional surfaces. Specifically, methods for live time-lapse imaging of T cell random migration and arrest on ICAM-1-coated surfaces are described. Additionally, we detail methods for live imaging of T-cell motility within 3D substrates to analyze T cell-antigen-presenting cell (APC) interactions and APC-mediated stop signals.",
keywords = "Arrest, Conjugation, D10 T cell, Immune synapse, Integrin, Leukocyte function-associated antigen-1 (LFA-1), Leukocyte, lymphocyte, Microscopy, Stop signal, T cell",
author = "Wiemer, \{Andrew J.\} and Sarah Wernimont and Anna Huttenlocher",
year = "2011",
doi = "10.1007/978-1-61779-166-6\_13",
language = "English (US)",
isbn = "9781617791659",
series = "Methods in Molecular Biology",
pages = "191--204",
editor = "Motomu Shimaoka and Motomu Shimaoka",
booktitle = "Integrin and Cell Adhesion Molecules",
}