Live imaging of LFA-1-dependent T-cell motility and stop signals

Andrew J. Wiemer, Sarah Wernimont, Anna Huttenlocher

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

T-cell motility is critical for leukocyte trafficking both in normal host defense and in pathologic conditions including chronic inflammatory disease. Despite progress in understanding the mechanisms of T-cell polarity and motility, we have limited understanding of the mechanisms that contribute to antigen-induced T cell arrest. Here, we describe methods to analyze leukocyte function antigen-1-mediated T-cell motility and T-cell receptor-induced stop signals using in vitro assays on two-dimensional surfaces. Specifically, methods for live time-lapse imaging of T cell random migration and arrest on ICAM-1-coated surfaces are described. Additionally, we detail methods for live imaging of T-cell motility within 3D substrates to analyze T cell-antigen-presenting cell (APC) interactions and APC-mediated stop signals.

Original languageEnglish (US)
Title of host publicationIntegrin and Cell Adhesion Molecules
Subtitle of host publicationMethods and Protocols
EditorsMotomu Shimaoka, Motomu Shimaoka
Pages191-204
Number of pages14
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume757
ISSN (Print)1064-3745

Keywords

  • Arrest
  • Conjugation
  • D10 T cell
  • Immune synapse
  • Integrin
  • Leukocyte function-associated antigen-1 (LFA-1)
  • Leukocyte, lymphocyte
  • Microscopy
  • Stop signal
  • T cell

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