Live-Attenuated Oral Vaccines to Reduce Campylobacter Colonization in Poultry

Byeonghwa Jeon, Tunchanok Saisom, Jiroj Sasipreeyajan, Taradon Luangtongkum

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The control of Campylobacter in poultry at the pre-harvest level is critical to reducing foodborne infections with Campylobacter since the consumption of contaminated poultry is the most frequent cause of human campylobacteriosis. Although poultry vaccination is suggested as useful intervention measures, no Campylobacter vaccines are currently available. To develop live-attenuated oral Campylobacter vaccines, in this study, we evaluated the efficacy of pre-colonization by oxidative stress defense mutants, including knockout mutants of ahpC, katA, and sodB, in preventing Campylobacter jejuni from colonizing poultry. Interestingly, when chickens were pre-colonized with ΔahpC and ΔkatA mutants, rather than the ΔsodB mutant, the level of C. jejuni colonization was significantly reduced within 35 days. Further studies demonstrated when chickens were pre-colonized with the ΔahpC mutant by oral challenge with a high dose (ca., 5 × 10 8 CFU/bird) and a low dose (ca., 5 × 10 6 CFU/bird), it twice reduced the level of C. jejuni by 3.9 log 10CFU/g feces and 3 log 10CFU/g feces after 42 days, respectively, compared to the untreated control. Due to a colonization defect, the ΔahpC mutant was removed from chickens within 42 days. After excretion from the host, moreover, the ΔahpC mutant cannot survive in aerobic environments because of compromised aerotolerance. Our findings suggest that the ahpC mutant has a great potential for on-farm application to control C. jejuni at the pre-harvest level.

Original languageEnglish (US)
Article number685
Issue number5
StatePublished - May 2022

Bibliographical note

Funding Information:
Funding: This research was funded by Alberta Livestock and Meat Agency (2013R039R) and the Canadian Poultry Research Council (AMN030).

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.


  • Campylobacter
  • food safety
  • live-attenuated oral vaccines
  • poultry

PubMed: MeSH publication types

  • Journal Article


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