Liquid chromatographic determination of reduced haloperidol and haloperidol concentrations in packed red blood cells from humans

G. T. Vatassery, L. A. Herzan, Maurice W Dysken

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Haloperidol (H) is a neuroleptic drug that has one known biologically active metabolite, reduced haloperidol (RH). A liquid chromatographic method is described for the determination of both compounds in human red blood cell (RBC) samples. The drugs were extracted into hexane at high pH and back-extracted into 0.1M HCI. The acid solution was then analyzed by reversed-phase chromatography under the following conditions: column was ultrasphere ODS; eluant was acetonitrile:0.085M phoehate buffer (30:70), final pH was 3.5, flow rate was 2 mL/min; detection was by light absorption at 246 nm for H and 220 nm for RH. The minimum limits of quantitation for H and RH were 0.25 and 0.1 ng/mL of packed RBC respectively. For six selected patients on 10 or 20 mg per day of oral haloperidol the RBC to plasma concentration ratios for RH and H were 2.20 ± 0.9 (SD) and 0.81 ± 0.26, respectively. The data indicate that RH is more concentrated in RBC than in plasma.

Original languageEnglish (US)
Pages (from-to)25-28
Number of pages4
JournalJournal of Analytical Toxicology
Volume14
Issue number1
DOIs
StatePublished - Jan 1990

Bibliographical note

Funding Information:
These studies were supported by research funds from the Veterans Administration.

Fingerprint

Dive into the research topics of 'Liquid chromatographic determination of reduced haloperidol and haloperidol concentrations in packed red blood cells from humans'. Together they form a unique fingerprint.

Cite this