Abstract
Haloperidol (H) is a neuroleptic drug that has one known biologically active metabolite, reduced haloperidol (RH). A liquid chromatographic method is described for the determination of both compounds in human red blood cell (RBC) samples. The drugs were extracted into hexane at high pH and back-extracted into 0.1M HCI. The acid solution was then analyzed by reversed-phase chromatography under the following conditions: column was ultrasphere ODS; eluant was acetonitrile:0.085M phoehate buffer (30:70), final pH was 3.5, flow rate was 2 mL/min; detection was by light absorption at 246 nm for H and 220 nm for RH. The minimum limits of quantitation for H and RH were 0.25 and 0.1 ng/mL of packed RBC respectively. For six selected patients on 10 or 20 mg per day of oral haloperidol the RBC to plasma concentration ratios for RH and H were 2.20 ± 0.9 (SD) and 0.81 ± 0.26, respectively. The data indicate that RH is more concentrated in RBC than in plasma.
Original language | English (US) |
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Pages (from-to) | 25-28 |
Number of pages | 4 |
Journal | Journal of Analytical Toxicology |
Volume | 14 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1990 |
Bibliographical note
Funding Information:These studies were supported by research funds from the Veterans Administration.