Liquid chromatographic assay of ketoconazole

F. A. Andrews, L. R. Peterson, W. H. Beggs, D. Crankshaw, G. A. Sarosi

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A reverse-phase, high-pressure liquid chromatographic method for the rapid and quantitative determination of ketoconazole has been developed. Drug levels from 0.5 to 10 μg/ml can be determined in either yeast nitrogen base medium or human serum by using an octadecylsilane column. A retention time of 4.9 ± 0.1 min resulted when the drug was eluted from a column with 75% methanol-25% 0.02 M (pH 7.5) phosphate buffer at a flow rate of 2 ml/min. Optimum sensitivity was obtained at a wavelength of 231 nm.

Original languageEnglish (US)
Pages (from-to)110-113
Number of pages4
JournalUnknown Journal
Volume19
Issue number1
DOIs
StatePublished - 1981

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