Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Orion M. Venero; Wakana Sato; Joseph M Heili; Christopher Deich; Katarzyna P. Adamala

doi:10.1007/978-1-0716-1998-8_14

Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Orion M. Venero, Wakana Sato, Joseph M Heili, Christopher Deich, Katarzyna P. Adamala

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Liposomal encapsulation serves as the basis for the engineering of biomimetic and novel synthetic cells. Liposomes are normally formed using such methods as thin film rehydration (TFH), density-mediated reverse emulsion encapsulation (REE), or one of many microfluidics-based approaches—with the latter of these two methods being used mainly for the encapsulation of various lumen constituents such as cell-free protein expression reactions. Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	227-235
Number of pages	9
Volume	2433
DOIs	https://doi.org/10.1007/978-1-0716-1998-8_14
State	Published - 2022

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work was supported by the John Templeton Foundation grant “Exploring the Informational Transitions Bridging Inorganic Chemistry and Minimal Life” and NSF Award 1844313. Wakana Sato was supported by the Funai Overseas Scholarship of The Funai Foundation for Information Technology, and Joseph Heili was supported by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate (NDSEG) Fellowship Program. We thank Annie Bonde for help with preparation of stock solutions and Christian Lysholm for advice regarding mechanical engineering.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

3D-printable
Giant unilamellar vesicles
Liposomal encapsulation
Microfluidics
Synthetic cells

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

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10.1007/978-1-0716-1998-8_14

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abstract = "Liposomal encapsulation serves as the basis for the engineering of biomimetic and novel synthetic cells. Liposomes are normally formed using such methods as thin film rehydration (TFH), density-mediated reverse emulsion encapsulation (REE), or one of many microfluidics-based approaches—with the latter of these two methods being used mainly for the encapsulation of various lumen constituents such as cell-free protein expression reactions. Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method.",

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ER -

Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Abstract

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