Liposomal encapsulation serves as the basis for the engineering of biomimetic and novel synthetic cells. Liposomes are normally formed using such methods as thin film rehydration (TFH), density-mediated reverse emulsion encapsulation (REE), or one of many microfluidics-based approaches—with the latter of these two methods being used mainly for the encapsulation of various lumen constituents such as cell-free protein expression reactions. Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||9|
|State||Published - 2022|
|Name||Methods in molecular biology (Clifton, N.J.)|
Bibliographical noteFunding Information:
This work was supported by the John Templeton Foundation grant “Exploring the Informational Transitions Bridging Inorganic Chemistry and Minimal Life” and NSF Award 1844313. Wakana Sato was supported by the Funai Overseas Scholarship of The Funai Foundation for Information Technology, and Joseph Heili was supported by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate (NDSEG) Fellowship Program. We thank Annie Bonde for help with preparation of stock solutions and Christian Lysholm for advice regarding mechanical engineering.
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Giant unilamellar vesicles
- Liposomal encapsulation
- Synthetic cells
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.