Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus

Orion M. Venero, Wakana Sato, Joseph M Heili, Christopher Deich, Katarzyna P. Adamala

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Liposomal encapsulation serves as the basis for the engineering of biomimetic and novel synthetic cells. Liposomes are normally formed using such methods as thin film rehydration (TFH), density-mediated reverse emulsion encapsulation (REE), or one of many microfluidics-based approaches—with the latter of these two methods being used mainly for the encapsulation of various lumen constituents such as cell-free protein expression reactions. Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by the John Templeton Foundation grant “Exploring the Informational Transitions Bridging Inorganic Chemistry and Minimal Life” and NSF Award 1844313. Wakana Sato was supported by the Funai Overseas Scholarship of The Funai Foundation for Information Technology, and Joseph Heili was supported by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate (NDSEG) Fellowship Program. We thank Annie Bonde for help with preparation of stock solutions and Christian Lysholm for advice regarding mechanical engineering.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • 3D-printable
  • Giant unilamellar vesicles
  • Liposomal encapsulation
  • Microfluidics
  • Synthetic cells

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.


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