Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains

Gunter P. Eckert, Urule Igbavboa, Walter E. Müller, W. Gibson Wood

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Lipid rafts have been proposed to be important in a variety of functions including lipid transport, signal transduction and cell growth. There is increasing evidence that lipid rafts may play a role in cell functions in brain. Lipid rafts are typically isolated using a detergent such as Triton X-100. There has been, however, data from non-brain tissue indicating that preparation of lipid rafts using a detergent may represent different raft domains as compared with non-detergent preparation. The purpose of the present study was to compare protein and lipid markers of lipid rafts using a highly purified mouse synaptosomal fraction and non-detergent and detergent methods. The lipid raft marker proteins, alkaline phosphatase and flotillin, and the lipid markers, cholesterol and sphingomyelin, were highly enriched in lipid rafts prepared with detergent as compared with the non-detergent fraction. Enrichment of Na+,K+-ATPase was greater in the non-detergent lipid raft fraction as compared with lipid rafts prepared with detergent. Lipid rafts from the nerve terminal of neurons prepared with or without detergents may represent different membrane domains each with unique specialized functions.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalBrain Research
Volume962
Issue number1-2
DOIs
StatePublished - Feb 7 2003

Bibliographical note

Funding Information:
This study was supported by NIH grant (1P0AG-18357), NATO Collaborative Linkage Grant (976648) and the Medical Research Program of the Department of Veterans Affairs.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

  • Alzheimer's disease
  • Cholesterol
  • Lipid raft
  • Membrane domain
  • Na,K-ATPase
  • Sphingomyelin

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