Linking physiological and genetic analyses of the control of leaf growth under changing environmental conditions

Decrease in leaf growth rate under water deficit can be seen as an adaptive process. The analysis of its genetic variability is therefore important in the context of drought tolerance. Several mechanisms are widely believed to drive the reduction in leaf growth rate under water deficit, namely leaf carbon balance, incomplete turgor maintenance, and decrease in cell wall plasticity or in cell division rate, with contributions from hormones such as abscisic acid or ethylene. Each of these mechanisms is still controversial, and involves several families of genes. It is argued that gene regulatory networks are not feasible for modelling such complex systems. Leaf growth can be modelled via response curves to environmental conditions, which are considered as 'meta-mechanisms' at a higher degree of organisation. Response curves of leaf elongation rate to meristem temperature, atmospheric vapour pressure deficit, and soil water status were established in recombinant inbred lines (RILs) of maize in experiments carried out in the field and in the greenhouse. A quantitative trait locus (QTL) analysis was conducted on the slopes of these responses. Each parameter of the ecophysiological model could then be computed as the sum of QTL effects, allowing calculation of parameters of new RILs, either virtual or existing. Leaf elongation rates of new RILS were simulated and were similar to measurements in a growth chamber experiment. This opens the way to the simulation of virtual genotypes, known only by their alleles, in any climatic scenario. Each genotype is therefore represented by a set of response parameters, valid in a large range of conditions and deduced from the alleles at QTLs.