@inproceedings{9843d36021f343e1b07675fcc3d7ee79,
title = "Linear micro-actuation system for patch-clamp recording",
abstract = "Measuring the electrical activity of neurons is essential for understanding how they encode and transmit information in the brain. Using a technique known as patch-clamping, the electrical activity of single neurons can be reliably recorded by pressing a small glass pipette filled with electrically conductive and pneumatically controlled solution against the neuron's membrane. This requires accurate and repeatable mechanical control of pipette position, typically necessitating a bulky actuation system and thus making it difficult to position several pipettes around a tissue specimen to record from multiple neurons at once. We have developed a linear micro-actuation system for patch-clamping that exhibits high positional accuracy (< 150 μm on-axis error over full travel), high repeatability (on-axis σ = 33 μm for full travel; σ = 0.71 μm for 15 μm travel) and low drift (0.61 μm/hour). The system was designed and fabricated to patch-clamp onto neurons in a mouse brain slice. The miniaturized device presented here makes it possible to position up to 21 actuators around a 5 × 5 mm tissue sample and eventually record intracellularly from a large number of neurons.",
author = "Ilya Kolb and Holst, {Gregory L.} and Stockslager, {Max A.} and Kodandaramaiah, {Suhasa B.} and William Stoy and Boyden, {Edward S.} and Forest, {Craig R.}",
year = "2014",
month = jan,
day = "1",
language = "English (US)",
series = "Proceedings - ASPE 2014 Annual Meeting",
publisher = "American Society for Precision Engineering, ASPE",
pages = "215--219",
booktitle = "Proceedings - ASPE 2014 Annual Meeting",
note = "29th Annual Meeting of the American Society for Precision Engineering, ASPE 2014 ; Conference date: 09-11-2014 Through 14-11-2014",
}