Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement

G. W.Gant Luxton, Edgar R. Gomes, Eric S. Folker, Erin Vintinner, Gregg G. Gundersen

Research output: Contribution to journalArticle

273 Scopus citations

Abstract

Nuclei move to specific locations to polarize migrating and differentiating cells. Many nuclear movements are microtubule-dependent. However, nuclear movement to reorient the centrosome in migrating fibroblasts occurs through an unknown actin-dependent mechanism. We found that linear arrays of outer (nesprin2G) and inner (SUN2) nuclear membrane proteins assembled on and moved with retrogradely moving dorsal actin cables during nuclear movement in polarizing fibroblasts. Inhibition of nesprin2G, SUN2, or actin prevented nuclear movement and centrosome reorientation. The coupling of actin cables to the nuclear membrane for nuclear movement via specific membrane proteins indicates that, like plasma membrane integrins, nuclear membrane proteins assemble into actin-dependent arrays for force transduction.

Original languageEnglish (US)
Pages (from-to)956-959
Number of pages4
JournalScience
Volume329
Issue number5994
DOIs
StatePublished - Aug 20 2010

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    Luxton, G. W. G., Gomes, E. R., Folker, E. S., Vintinner, E., & Gundersen, G. G. (2010). Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement. Science, 329(5994), 956-959. https://doi.org/10.1126/science.1189072