Light-Scattering Characteristics of Biological Particles

Dennis K. Kreid, Richard J Goldstein, Marvin R. Kammin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Measurements have been obtained of the light-scattering characteristics of 0.5 μ diameter polystyrene spheres, red blood cells, and hemoglobin free red cell “ghosts.” The information obtained consists of measurements of the magnitude of laser radiation scattered by dilute aqueous suspensions of the above particles as a function of the particle concentration and the angle of observation, measured from the direction of the incident beam. In addition, the results for the spheres are compared to analytical predictions calculated from the Mie theory. The light source employed is a 75 mW He Ne laser operating continuously at 632.8 nm. The laser beam is spatially filtered and focused (large F no.) at the center of a 1.6 cm i.d. cylindrical glass scattering cell. Scattered intensity measurements are obtained with an S-20 multiplier phototube, mounted on a beam which rotates about the scattering cell as an axis.

Original languageEnglish (US)
Pages (from-to)521-541
Number of pages21
JournalBiomaterials, Medical Devices, and Artificial Organs
Volume1
Issue number3
DOIs
StatePublished - Jan 1 1973

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