Light-evoked NMDA receptor-mediated currents are reduced by blocking D-serine synthesis in the salamander retina

Eric R. Stevens, Eric C. Gustafson, Steven J. Sullivan, Manuel Esguerra, Robert F. Miller

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Experiments were carried out in the retina of the tiger salamander (Ambystoma tigrinum) to evaluate the importance of D-serine synthesis on light-evoked N-methyl D-aspartate (NMDA) receptor-mediated components of ganglion cells and contributions to the proximal negative field potential. We blocked the synthesis of D-serine through brief exposures of the retina to phenazine ethosulfate and validated the changes in the tissue levels of D-serine using capillary electrophoresis methods to separate and measure the amino acid enantiomers. Ten minute exposures to phenazine ethosulfate decreased D-serine levels in the retina by about 50% and significantly reduced the NMDA receptor contribution to light responses of the inner retina. This is the first report of a linkage between D-serine synthesis and NMDA receptor activity in the vertebrate retina.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalNeuroreport
Volume21
Issue number4
DOIs
StatePublished - Mar 2010

Keywords

  • D-serine
  • Ganglion cells
  • Glia
  • NMDA receptors
  • Retina
  • Serine racemase

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